US 12,240,885 B2
Peptide-MHC compacts
Olivier Dalmas, San Carlos, CA (US); Zheng Pan, Freemont, CA (US); Michael Bethune, Castro Valley, CA (US); and Songming Peng, San Mateo, CA (US)
Assigned to ADOC SSF, LLC, South San Francisco, CA (US)
Filed by ADOC SSF, LLC, South San Francisco, CA (US)
Filed on Nov. 18, 2020, as Appl. No. 16/951,925.
Application 16/951,925 is a division of application No. 16/679,025, filed on Nov. 8, 2019, granted, now 10,875,905.
Application 16/679,025 is a continuation of application No. PCT/US2019/025415, filed on Apr. 2, 2019.
Claims priority of provisional application 62/651,639, filed on Apr. 2, 2018.
Prior Publication US 2021/0070834 A1, Mar. 11, 2021
Int. Cl. C07K 14/74 (2006.01); C07K 14/61 (2006.01); C12N 15/10 (2006.01); G01N 33/569 (2006.01)
CPC C07K 14/70539 (2013.01) [C07K 14/61 (2013.01); C12N 15/1068 (2013.01); G01N 33/56972 (2013.01); C07K 2319/02 (2013.01); C07K 2319/20 (2013.01); C07K 2319/21 (2013.01); C07K 2319/50 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for isolating an antigen-specific T cell, the method comprising:
a. preparing a plurality of particles by
i) obtaining a library comprising at least two polynucleotide, each polynucleotide comprising, from 5′ to 3′, a sequence insert comprising a stop codon in each of the three reading frames of the polynucleotide, a Beta 2 Microglobulin (β2M) coding sequence, and a Major Histocompatibility Complex (MHC) heavy chain coding sequence, wherein the sequence insert is flanked at its 5′ or 3′ by a first universal target sequence comprising a restriction site or a polymerase chain reaction (PCR) primer target site;
ii) inserting a polynucleotide encoding an antigenic sequence into the sequence insert, to thereby obtain a polypeptide comprising, in an amino to carboxyl terminus orientation, the antigenic sequence, the Beta 2 Microglobulin (β2M) sequence, and the Major Histocompatibility Complex (MHC) heavy chain sequence;
iii) attaching the polypeptide to a particle to obtain a plurality of particles;
b. contacting the plurality of particles with a plurality of T cells under conditions suitable for antigen-specific binding of a T cell to a particle; and
c. isolating the particles, to thereby isolate an antigen-specific T cell bound thereto;
wherein the particle is a magnetic bead, an agarose bead, a styrene polymer particle, or a dextran polymer particle.