US 12,240,770 B2
Systems and method for correction of positionally dependent electromagnetic radiation detected from objects within a fluid column
Peter Kiesel, Palo Alto, CA (US); Todd Karin, Berkeley, CA (US); and Kenneth Michael Evans, College Station, TX (US)
Assigned to INGURAN, LLC, Navasota, TX (US)
Filed by Inguran, LLC, Navasota, TX (US)
Filed on Dec. 29, 2023, as Appl. No. 18/400,750.
Application 18/400,750 is a continuation of application No. 18/099,137, filed on Jan. 19, 2023, granted, now 11,858,832.
Application 18/099,137 is a continuation of application No. 16/820,585, filed on Mar. 16, 2020, granted, now 11,584,662, issued on Feb. 21, 2023.
Prior Publication US 2024/0140830 A1, May 2, 2024
Int. Cl. C02F 1/32 (2023.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01); G01N 15/1404 (2024.01); G01N 15/1429 (2024.01); G01N 15/1434 (2024.01); G01N 21/64 (2006.01); G01N 33/50 (2006.01)
CPC C02F 1/325 (2013.01) [G01N 15/14 (2013.01); G01N 15/1404 (2013.01); G01N 15/1429 (2013.01); G01N 15/1434 (2013.01); G01N 15/1436 (2013.01); G01N 15/1459 (2013.01); G01N 21/645 (2013.01); G01N 2015/1006 (2013.01); G01N 2021/6463 (2013.01); G01N 21/6486 (2013.01); G01N 33/5005 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A discrimination system, comprising:
collection optics that collect output electromagnetic radiation from X-chromosome bearing sperm and Y-chromosome bearing sperm within a measurement region;
a detector that generates an electrical signal responsive to the intensity of the output electromagnetic radiation collected by the collection optics; and
an analyzer having instructions stored thereon i) to normalize the intensity of the output electromagnetic radiation represented in the electrical signal based on the position of the sperm cells in the measurement region, and ii) to discriminate between X-chromosome bearing sperm and Y-chromosome bearing sperm.