US 12,239,973 B2
Particle-drop structures and methods for making and using the same
Dino Di Carlo, Los Angeles, CA (US); and Chueh-Yu Wu, Los Angeles, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed on Sep. 30, 2024, as Appl. No. 18/901,981.
Application 18/901,981 is a continuation of application No. 18/161,322, filed on Jan. 30, 2023.
Application 18/161,322 is a continuation of application No. 16/550,105, filed on Aug. 23, 2019, granted, now 11,590,489, issued on Feb. 28, 2023.
Application 16/550,105 is a continuation in part of application No. PCT/US2018/019486, filed on Feb. 23, 2018.
Claims priority of provisional application 62/463,272, filed on Feb. 24, 2017.
Prior Publication US 2025/0018386 A1, Jan. 16, 2025
Int. Cl. B01L 3/02 (2006.01); B01L 3/00 (2006.01); C12N 1/04 (2006.01); C12N 5/071 (2010.01); C12N 5/0783 (2010.01); C12Q 1/6806 (2018.01); G01N 33/543 (2006.01)
CPC B01L 3/0262 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502784 (2013.01); C12N 1/04 (2013.01); C12N 5/0625 (2013.01); C12N 5/0636 (2013.01); C12Q 1/6806 (2013.01); G01N 33/5432 (2013.01); B01L 2300/021 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0896 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method of performing a single-cell analysis comprising:
(a) forming a first aqueous solution comprising a plurality of cells and a plurality of three-dimensional particles, wherein each three-dimensional particle of the plurality of three-dimensional particles comprises a unique barcode, by mixing the plurality of three-dimensional particles and an aqueous sample comprising the plurality of cells;
(b) generating a plurality of aqueous drops, each aqueous drop of the plurality of aqueous drops containing a single three-dimensional particle and a single cell by emulsifying the first aqueous solution with an oil phase by using a mixing force;
(c) generating released nucleic acid molecules by releasing nucleic acid molecules from at least one single cell; and
(d) performing the single-cell analysis by analyzing the released nucleic acid molecules.