US 12,239,738 B2
Compositions and methods for lyophilization of bacteria or listeria strains
David Fela, Califon, NJ (US); Anu Wallecha, Yardley, PA (US); Mike Grace, Monmouth Junction, NJ (US); and Melissa Gosse, Jackson, NJ (US)
Assigned to Advaxis, Inc., Monmouth Junction, NJ (US)
Filed by Advaxis, Inc., Monmouth Junction, NJ (US)
Filed on Oct. 18, 2021, as Appl. No. 17/503,647.
Application 17/503,647 is a division of application No. 16/647,977, granted, now 11,179,339, previously published as PCT/US2018/048586, filed on Aug. 29, 2018.
Claims priority of provisional application 62/560,318, filed on Sep. 19, 2017.
Prior Publication US 2022/0062181 A1, Mar. 3, 2022
Int. Cl. A61K 9/19 (2006.01); A61K 35/74 (2015.01); C12N 1/20 (2006.01)
CPC A61K 9/19 (2013.01) [A61K 35/74 (2013.01); C12N 1/20 (2013.01)] 20 Claims
 
1. A formulation for lyophilization of a Listeria strain, comprising: (1) the Listeria strain; (2) a phosphate buffer; and (3) sucrose,
wherein the Listeria strain is a recombinant Listeria monocytogenes strain,
wherein the formulation comprises 1% to 5% w/v sucrose, and
wherein the formulation does not comprise trehalose, monosodium glutamate (MSG), or recombinant human serum albumin (rHSA).