| CPC A61K 9/1271 (2013.01) [A61K 31/517 (2013.01); A61K 47/26 (2013.01); A61K 31/282 (2013.01); C07K 16/2818 (2013.01)] | 21 Claims |

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1. A liposomal composition comprising a gamma polyglutamated raltitrexed encapsulated by a liposome, wherein the gamma polyglutamated raltitrexed contains 2-10 glutamyl groups having gamma carboxy group linkages, wherein the liposome is pegylated and does not contain a targeting moiety having specific affinity for a surface antigen on a target cell,
wherein the liposome does not contain a cell penetrating peptide and does not contain a mitochondria penetrating agent,
wherein the liposome has a zeta potential that is less than or equal to zero, between 0 and −150 mV, or between −30 and −50 mV, and
wherein the liposome is capable of delivering the gamma polyglutamated raltitrexed directly into a cell.
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