	US 12,239,718 B2
	Synthetic cellular signaling pathways and uses thereof
Mark W. Grinstaff, Brookline, MA (US); Jack R. Kirsch, Cambridge, MA (US); Amanda K. Williamson, Boston, MA (US); and Brett Tingley, Brighton, MA (US)
Assigned to Trustees of Boston University
Filed by Trustees of Boston University, Boston, MA (US)
Filed on Jul. 12, 2023, as Appl. No. 18/221,206.
Claims priority of provisional application 63/388,343, filed on Jul. 12, 2022.
Prior Publication US 2024/0024505 A1, Jan. 25, 2024
Int. Cl. A61K 48/00 (2006.01); A61K 9/16 (2006.01); A61K 38/22 (2006.01); A61K 38/28 (2006.01)

CPC A61K 48/0033 (2013.01) [A61K 9/1617 (2013.01); A61K 38/2221 (2013.01); A61K 38/28 (2013.01)]

16 Claims

1. A nucleic acid combination comprising:

(i) a first nucleic acid molecule encoding a ligand; and

(ii) a second nucleic acid molecule encoding a target molecule, wherein the target is a cognate receptor of the ligand or an inhibitor of a suppressor of the ligand,

wherein one of the ligand and the target molecule is translated via a cap-dependent manner and the other of the ligand and the target molecule is translated via a cap-independent manner,

wherein the first and second nucleic acid are covalently linked to form a nucleic acid encoding both the ligand and the target molecule,

wherein the ligand is a protein, a protein fragment, a fusion protein, and wherein the target molecule is a protein or a polypeptide.