US 12,239,706 B2
Method and system for protecting monarch butterflies from pesticides
Joseph E. Kovarik, Englewood, CO (US)
Assigned to Seed Health, Inc., Venice, CA (US)
Filed by Seed Health, Inc., Venice, CA (US)
Filed on Feb. 23, 2022, as Appl. No. 17/678,840.
Application 17/678,840 is a continuation in part of application No. 17/185,618, filed on Feb. 25, 2021, granted, now 11,529,412.
Application 17/185,618 is a continuation in part of application No. 16/892,623, filed on Jun. 4, 2020, granted, now 10,933,128, issued on Mar. 2, 2021.
Application 16/892,623 is a continuation in part of application No. 16/246,652, filed on Jan. 14, 2019, granted, now 10,675,347, issued on Jun. 9, 2020.
Application 16/246,652 is a continuation in part of application No. 16/139,232, filed on Sep. 24, 2018, granted, now 10,568,916, issued on Feb. 25, 2020.
Application 16/139,232 is a continuation in part of application No. 15/379,579, filed on Dec. 15, 2016, granted, now 10,086,024, issued on Oct. 2, 2018.
Application 17/185,618 is a continuation in part of application No. 15/270,034, filed on Sep. 20, 2016, granted, now 9,750,802, issued on Sep. 5, 2017.
Application 15/270,034 is a continuation of application No. 14/954,074, filed on Nov. 30, 2015, granted, now 9,457,077, issued on Oct. 4, 2016.
Claims priority of provisional application 62/278,046, filed on Jan. 13, 2016.
Claims priority of provisional application 62/277,571, filed on Jan. 12, 2016.
Prior Publication US 2022/0175914 A1, Jun. 9, 2022
Int. Cl. A61K 39/35 (2006.01); A61K 9/00 (2006.01); A61K 35/74 (2015.01); A61K 35/741 (2015.01); A61K 35/742 (2015.01); A61K 35/745 (2015.01); A61K 35/747 (2015.01); A61K 39/00 (2006.01); A61K 39/39 (2006.01); A61K 39/395 (2006.01); A61K 45/06 (2006.01); C07K 16/00 (2006.01)
CPC A61K 39/35 (2013.01) [A61K 9/0034 (2013.01); A61K 9/0043 (2013.01); A61K 9/007 (2013.01); A61K 35/74 (2013.01); A61K 35/741 (2013.01); A61K 35/742 (2013.01); A61K 35/745 (2013.01); A61K 35/747 (2013.01); A61K 39/0008 (2013.01); A61K 39/39 (2013.01); A61K 39/395 (2013.01); A61K 45/06 (2013.01); C07K 16/00 (2013.01); A61K 2039/505 (2013.01); A61K 2039/541 (2013.01); A61K 2039/544 (2013.01); A61K 2039/55 (2013.01); A61K 2039/55583 (2013.01); A61K 2039/577 (2013.01); A61K 2039/58 (2013.01); A61K 2039/70 (2013.01)] 20 Claims
 
1. A method for providing a Monarch butterfly with the ability to assimilate pesticides, comprising, inoculating a Monarch butterfly with a culture of pesticide degrading bacteria, wherein the pesticide degrading bacteria include genes whose expression by the pesticide degrading bacteria results in the degradation of the pesticide, said pesticide degrading bacteria being modified to include said genes using a clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR associated protein (Cas) system or using a clustered regularly interspaced short palindromic repeats (CRISPR) from prevotella and francisella 1 (Cpf1) nuclease, said pesticide degrading bacteria selected from the group consisting of L. rhamnosus and L. plantarum.