| CPC A61K 39/215 (2013.01) [A61K 2039/55505 (2013.01); A61K 2039/55561 (2013.01); A61K 2039/6087 (2013.01)] | 10 Claims |

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1. A nano-vaccine particle, comprising:
an amphiphilic carrier;
an active ingredient; and
at least one adjuvant,
wherein the nano-vaccine particle shows a negatively-charged surface, and
wherein the amphiphilic carrier encapsulates the active ingredient and the adjuvant,
wherein the active ingredient is a spike receptor-binding domain protein of SARS-CoV-2, and
wherein an amino acid sequence of the spike receptor-binding domain protein is set forth by SEQ ID NO:1.
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