	US 12,239,697 B2
	Virus-like particle conjugates
Subhash V. Kapre, Redmond, WA (US); and Anup K. Datta, Renton, WA (US)
Assigned to Inventprise, Inc., Redmond, WA (US)
Filed by Inventprise, LLC, Redmond, WA (US)
Filed on Jul. 5, 2022, as Appl. No. 17/857,303.
Application 17/857,303 is a continuation of application No. 16/435,502, filed on Jun. 9, 2019, granted, now 11,389,519.
Claims priority of provisional application 62/683,787, filed on Jun. 12, 2018.
Claims priority of provisional application 62/683,543, filed on Jun. 11, 2018.
Prior Publication US 2023/0093700 A1, Mar. 23, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/12 (2006.01); A61K 39/108 (2006.01); A61K 39/39 (2006.01); A61K 47/65 (2017.01); C07K 14/025 (2006.01); C12N 7/00 (2006.01)

CPC A61K 39/0258 (2013.01) [A61K 39/12 (2013.01); A61K 39/39 (2013.01); A61K 47/65 (2017.08); C07K 14/025 (2013.01); C12N 7/00 (2013.01); C12N 2710/20023 (2013.01)]

30 Claims

1. A method for manufacture of an immunogenic composition comprising:

coupling a first set of L1 or L2 proteins of Human papilloma virus (HPV) to a first set of heterobifunctional, homobifunctional, or multifunctional spacer arms forming a first set of coupled L1 or L2 proteins;

coupling a second set of L1 or L2 proteins of HPV to a second set of heterobifunctional, homobifunctional, or multifunctional spacer arms forming a second set of coupled L1 or L2 proteins; and

coupling each of the first and second sets of coupled L1 or L2 proteins to a carrier protein forming the immunogenic composition.