US 12,239,682 B1
Peony stamen-dodder composite, and its preparation method and application
Wenpeng Yuan, Heze (CN); Dandan Cheng, Heze (CN); and Zhiqiang Huang, Heze (CN)
Assigned to HEZE BRANCH, QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES), Heze (CN)
Filed by HEZE BRANCH, QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES), Heze (CN)
Filed on Nov. 14, 2024, as Appl. No. 18/948,016.
Application 18/948,016 is a continuation of application No. PCT/CN2024/105571, filed on Jul. 15, 2024.
Claims priority of application No. 202310896726.7 (CN), filed on Jul. 20, 2023.
Int. Cl. A61K 36/00 (2006.01); A61K 36/39 (2006.01); A61K 36/65 (2006.01); A61P 13/12 (2006.01)
CPC A61K 36/65 (2013.01) [A61K 36/39 (2013.01); A61P 13/12 (2018.01); A61K 2236/15 (2013.01); A61K 2236/17 (2013.01); A61K 2236/331 (2013.01); A61K 2236/51 (2013.01); A61K 2236/53 (2013.01)] 5 Claims
 
1. A peony stamen-dodder composite for treating nephritis, wherein raw materials of the peony stamen-dodder composite comprise 10-18 parts by weight of a peony stamen and 5-12 parts by weight of a dodder; and the peony stamen-dodder composite is prepared through steps of:
step (1) subjecting the peony stamen to desensitization, impregnation with a first saline and drying, and subjecting the dodder to impregnation with a second saline and baking; and
step (2) crushing a treated peony stamen obtained in step (1), and crushing a treated dodder obtained in step (1); impregnating a crushed peony stamen and a crushed dodder with warm water followed by a first ultrasonic treatment and filtration to obtain a first filtrate and a filter residue; dispersing the filter residue in water followed by a second ultrasonic treatment and filtration to obtain a second filtrate; and combining the first filtrate with the second filtrate followed by vacuum concentration to obtain the peony stamen-dodder composite;
wherein in step (1), the desensitization is performed at 120° C. for 1-3 min, or performed by microwave-assisted desensitization at a power of 3,000-5,000 W and a temperature of 65-80° C. for 1-3 min; a thickness of the peony stamen is 3-6 mm; the drying is performed at 55-65° C. for 4.5-5.5 h; the baking is performed at 75-85° C. for 7.5-8.5 h; the first saline and the second saline both have a concentration of 1.2-1.8 wt. %; a weight of the first saline is 1-2 times a weight of the peony stamen; a weight of the second saline is 0.25-2 times the weight of the peony stamen; and the impregnation of the peony stamen is performed for 8-15 min, and the impregnation of the dodder is performed for 15-25 min;
in step (2), a weight of the warm water is 19-21 times a total weight of the peony stamen and the dodder, a temperature of the warm water is 65-75° C., and the crushed peony stamen and the crushed dodder are impregnated in the warm water for 1.5-2.5 h;
in step (2), the first ultrasonic treatment is carried out for 25-35 min, and the second ultrasonic treatment is carried out for 55-65 min; a weight of the water for dispersing the filter residue is 14-16 times a total weight of the peony stamen and the dodder; and
in step (2), a temperature of the vacuum concentration does not exceed 70° C., and the vacuum concentration is performed such that a weight of a concentrated product is 7.9-8.1 times the total weight of the peony stamen and the dodder.