| CPC A61K 35/28 (2013.01) [A61K 35/35 (2013.01); A61L 27/3691 (2013.01); B01D 21/0012 (2013.01); B01D 21/262 (2013.01); B01L 3/5021 (2013.01); B04B 3/00 (2013.01); B33Y 10/00 (2014.12); B33Y 70/00 (2014.12); A61L 2430/40 (2013.01); B01D 2221/10 (2013.01); B01L 2200/025 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0832 (2013.01)] | 21 Claims |

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1. A tissue processing device comprising:
a first centrifuge tube configured to receive and process a biological substance, the first centrifuge tube comprising an upper cylindrical portion and a lower conical portion, a sterile tissue inlet fitting, at least one sterile processing fluid inlet fitting, a sterile suction fitting, and at least one sterile extraction port connected to a first extraction tube;
wherein the first centrifuge tube further comprises an internal space including a screen being positioned therein, the screen being configured to provide support for the biological substance and comprising a plurality of pores being less than 1.2 mm, and a filter positioned therein, the filter being positioned below the screen in the lower conical portion of the first centrifuge tube; and
a second centrifuge tube configured to receive and further process the biological substance from the first centrifuge tube, the second centrifuge tube comprising at least one sterile fitting, wherein the second centrifuge tube is releasably connected via the at least one sterile fitting to the at least one sterile extraction port of the first centrifuge tube.
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