US 12,239,633 B2
Materials and methods for improving gastrointestinal function
Sadasivan Vidyasagar, Gainesville, FL (US); Paul Okunieff, Gainesville, FL (US); and Lurong Zhang, Gainesville, FL (US)
Assigned to University of Florida Research Foundation, Incorporated, Gainesville, FL (US)
Filed by University of Florida Research Foundation, Incorporated, Gainesville, FL (US)
Filed on Aug. 1, 2023, as Appl. No. 18/363,448.
Application 18/363,448 is a continuation of application No. 17/161,811, filed on Jan. 29, 2021, granted, now 11,752,132.
Application 17/161,811 is a continuation of application No. 16/441,062, filed on Jun. 14, 2019, granted, now 10,940,137, issued on Mar. 9, 2021.
Application 16/441,062 is a continuation of application No. 14/656,255, filed on Mar. 12, 2015, granted, now 10,322,109, issued on Jun. 18, 2019.
Application 14/656,255 is a continuation of application No. 13/245,430, filed on Sep. 26, 2011, granted, now 8,993,522, issued on Mar. 31, 2015.
Claims priority of provisional application 61/431,629, filed on Jan. 11, 2011.
Claims priority of provisional application 61/386,317, filed on Sep. 24, 2010.
Prior Publication US 2024/0024286 A1, Jan. 25, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 31/405 (2006.01); A61K 9/00 (2006.01); A61K 31/197 (2006.01); A61K 31/198 (2006.01); A61K 31/7004 (2006.01); A61K 31/7016 (2006.01); A61K 47/18 (2017.01); A61K 47/20 (2006.01); A61P 1/12 (2006.01)
CPC A61K 31/405 (2013.01) [A61K 9/0095 (2013.01); A61K 31/197 (2013.01); A61K 31/198 (2013.01); A61K 31/7004 (2013.01); A61K 31/7016 (2013.01); A61K 47/183 (2013.01); A61K 47/20 (2013.01); A61P 1/12 (2018.01)] 21 Claims
 
1. A method for improving intestinal barrier function in a subject in need thereof, the method comprising:
administering a formulation comprising free amino acids to the subject in need thereof,
wherein the free amino acids comprise, as free amino acids, a therapeutically effective amount of serine and valine; and
a therapeutically effective amount of at least one additional free amino acid of aspartic acid, glycine, isoleucine, lysine, threonine, tryptophan, tyrosine, or any combination thereof;
wherein the therapeutically effective amount of the serine and valine and the therapeutically effective amount of the at least one additional free amino acid promotes intestinal health in the subject;
wherein the formulation does not comprise glucose, or when the formulation does comprise glucose, the glucose is present in a concentration equal to or less than 10 mM;
wherein the formulation does not comprise:
free amino acid glutamine;
a glutamine-containing dipeptide;
or any combination thereof;
wherein the formulation does not comprise riboflavin; and
wherein a therapeutically effective amount of the formulation improves intestinal barrier function in the subject.