US 12,239,613 B2
Preparation method for carotenoid preparation having high bioavailability and high stability
Xinde Xu, Shaoxing (CN); Di Zhou, Shaoxing (CN); Tian Xie, Shaoxing (CN); Shuangming Ye, Shaoxing (CN); and Shengnan Wang, Shaoxing (CN)
Assigned to ZHEJIANG MEDICINE CO., LTD. XINCHANG PHARMACEUTICAL FACTORY, Zhejiang (CN)
Appl. No. 17/440,198
Filed by ZHEJIANG MEDICINE CO., LTD. XINCHANG PHARMACEUTICAL FACTORY, Zhejiang (CN)
PCT Filed Mar. 10, 2020, PCT No. PCT/CN2020/078522
§ 371(c)(1), (2) Date Sep. 17, 2021,
PCT Pub. No. WO2020/187084, PCT Pub. Date Sep. 24, 2020.
Claims priority of application No. 201910205666.3 (CN), filed on Mar. 18, 2019.
Prior Publication US 2022/0160653 A1, May 26, 2022
Int. Cl. A61K 31/122 (2006.01); A61K 9/16 (2006.01); A61K 31/01 (2006.01); A61K 31/047 (2006.01); B02C 23/36 (2006.01)
CPC A61K 31/122 (2013.01) [A61K 9/1623 (2013.01); A61K 9/1652 (2013.01); A61K 9/1658 (2013.01); A61K 9/1694 (2013.01); A61K 31/01 (2013.01); A61K 31/047 (2013.01); B02C 23/36 (2013.01)] 9 Claims
 
1. A preparation method of carotenoid water-dispersible microcapsule with the preparation method comprising:
a) dissolving part of a water-soluble colloid and a filling substance in water to form an aqueous phase, wherein the water-soluble colloid is selected from the group consisting of gelatin, casein, and soy isolate protein, and wherein the filling substance is syrup;
b) adding a carotenoid crystal to the aqueous phase and stirring to form a dispersion liquid;
c) loading the dispersion liquid into a first-stage grinding machine for first grinding, wherein the first-stage grinding machine comprises a grinding medium made of beads, wherein the beads of the first-stage grinding machine have a bead size of 0.6-0.8 mm, performing the first grinding until particle size of the carotenoid crystal is 0.5-1.5 μm, and then stopping the first grinding to obtain a first grade grinding liquid;
d) adding the remaining water-soluble colloid and the filling substance to the first-stage grinding liquid, loading the first-stage grinding liquid with the remaining water-soluble colloid and the filling substance into a second-stage grinding machine for second grinding, wherein the second-stage grinding machine comprises a grinding medium made of beads, wherein the beads of the second-stage grinding machine have a bead size of 0.3-0.4 mm, performing the second grinding until the particle size of the carotenoid crystal is 150-600 nm, and then stopping the second grinding to obtain a second grade grinding liquid, wherein a total grinding time comprising the first grinding plus the second grinding is 0.5-2.0 hours; and
e) drying the moisture in the second-stage grinding liquid to obtain carotenoid dry powder or particles.