	US 12,239,124 B2
	Compositions for cryopreservation of a biological material
Fabien Ectors, Gesves (BE); Luc Grobet, Esneux (BE); Delphine Connan, Gesves (BE); and Nadine Dupuis, Burg-Reuland (BE)
Assigned to Cryoport Belgium S.A., Villers-le-Bouillet (BE)
Appl. No. 17/421,134
Filed by Cryoport Belgium S.A., Villers-le-Bouillet (BE)
PCT Filed Feb. 7, 2020, PCT No. PCT/EP2020/053065 § 371(c)(1), (2) Date Jul. 7, 2021, PCT Pub. No. WO2020/161273, PCT Pub. Date Aug. 13, 2020.
Claims priority of application No. 19155970 (EP), filed on Feb. 7, 2019.
Prior Publication US 2022/0217969 A1, Jul. 14, 2022
Int. Cl. A01N 1/02 (2006.01)

CPC A01N 1/0221 (2013.01)

13 Claims

1. A liquid composition for cryopreservation of a biological material comprising

a polar aprotic solvent selected from dimethylsulfoxide (DMSO) and dimethylformamide (DMF),

a monohydric or polyhydric alcohol selected from ethylene glycol, propylene glycol, glycerol, erythritol, sorbitol, mannitol, xylitol, volemitol, methanol, ethanol, isopropanol, butanol and pentanol;

an unbranched polysaccharide selected from the group consisting of sucrose, trehalose, lactulose, melibiose, lactobionate, raffinose and cellulose;

dextran; and

polyvinyl alcohol;

and wherein the dextran comprises Dextran 1, Dextran 40, and Dextran 70.