US 12,239,090 B2
Plant body of genus Nicotiana with low alkaloid content and production method thereof
Takanori Takeuchi, Tokyo (JP); Masao Arai, Tokyo (JP); Hisashi Udagawa, Tokyo (JP); Hiroshi Magome, Tokyo (JP); Ryo Nishiguchi, Tokyo (JP); and Yoshimitsu Takakura, Tokyo (JP)
Assigned to JAPAN TOBACCO INC., Tokyo (JP)
Filed by JAPAN TOBACCO INC., Tokyo (JP)
Filed on Oct. 13, 2022, as Appl. No. 17/965,450.
Application 17/965,450 is a continuation of application No. PCT/JP2021/015920, filed on Apr. 19, 2021.
Claims priority of application No. 2020-074233 (JP), filed on Apr. 17, 2020.
Prior Publication US 2023/0120622 A1, Apr. 20, 2023
Int. Cl. A01H 6/82 (2018.01); C12N 15/82 (2006.01)
CPC A01H 6/823 (2018.05) [C12N 15/8243 (2013.01)] 17 Claims
 
1. A tobacco plant in which a mutation that specifically causes suppression of a function of an endogenous gene is introduced in the endogenous gene in a genome, the endogenous gene being at least one of:
(a) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 5; and
(b) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO: 6; wherein respective functions of the following endogenous genes (c) and (d) are not suppressed:
(c) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO. 62 or SEQ ID NO: 63; and
(d) an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO. 64.