US 11,914,130 B2
Auto-focus methods and systems for multi-spectral imaging
Joerg Bredno, San Francisco, CA (US); Jim F. Martin, Mountain View, CA (US); and Anindya Sarkar, Milpitas, CA (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Aug. 31, 2022, as Appl. No. 17/900,127.
Application 17/900,127 is a division of application No. 16/931,319, filed on Jul. 16, 2020, granted, now 11,467,390.
Application 16/931,319 is a continuation of application No. 16/199,286, filed on Nov. 26, 2018, granted, now 10,768,403, issued on Sep. 8, 2020.
Application 16/199,286 is a continuation of application No. 14/901,700, abandoned, previously published as PCT/EP2014/065064, filed on Jul. 15, 2014.
Claims priority of provisional application 61/847,978, filed on Jul. 18, 2013.
Prior Publication US 2023/0114003 A1, Apr. 13, 2023
Int. Cl. G02B 21/24 (2006.01); G02B 21/00 (2006.01); G02B 21/16 (2006.01); G02B 21/36 (2006.01); G06T 7/80 (2017.01); G06V 20/69 (2022.01); H04N 23/10 (2023.01); H04N 23/67 (2023.01)
CPC G02B 21/244 (2013.01) [G02B 21/006 (2013.01); G02B 21/0072 (2013.01); G02B 21/008 (2013.01); G02B 21/367 (2013.01); G06T 7/80 (2017.01); G06V 20/69 (2022.01); H04N 23/10 (2023.01); H04N 23/67 (2023.01); G02B 21/16 (2013.01); G06T 2207/10056 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for acquiring focused images of a biological specimen disposed on a microscope slide using a microscope having an optical system and a digital color camera, the method comprising:
computing an adjusted focus plane value for each of a plurality of color channels of the digital color camera, wherein each adjusted focus plane value is computed at a plurality of slide positions for the biological specimen disposed on the slide, and wherein each adjusted focus plane value is computed from (i) an obtained in-focus focal base plane value for each of the plurality of slide positions, and (ii) offset values for the each of the plurality color channels at each of the plurality of slide positions;
characterizing chromatic aberration present in the optical system as a Z-offset based on the adjusted focus plane value in each of the plurality of color channels; and
scanning a microscope slide in a manner to reduce the effect of the characterized chromatic aberration by obtaining image data from the plurality of color channels in accordance with the Z-offsets.