	US 11,913,952 B2
	Methods for detecting peptide/MHC/TCR binding
John Andrew Altin, Flagstaff, AZ (US); and Mark S. Chee, Encinitas, CA (US)
Assigned to Prognosys Biosciences, Inc., San Diego, CA (US)
Filed by Prognosys Biosciences, Inc., San Diego, CA (US)
Filed on Nov. 22, 2021, as Appl. No. 17/532,728.
Application 17/532,728 is a continuation of application No. 14/776,537, granted, now 11,231,419, previously published as PCT/US2014/029691, filed on Mar. 14, 2014.
Claims priority of provisional application 61/800,891, filed on Mar. 15, 2013.
Prior Publication US 2022/0146513 A1, May 12, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/569 (2006.01); C12Q 1/6804 (2018.01); G01N 33/538 (2006.01); C07K 7/08 (2006.01)

CPC G01N 33/56977 (2013.01) [C07K 7/08 (2013.01); C12Q 1/6804 (2013.01); G01N 33/538 (2013.01); G01N 2333/003 (2013.01); G01N 2333/70539 (2013.01)]

19 Claims

1. A composition comprising a plurality of complexes,

wherein each complex comprises a conjugate and a major histocompatibility complex (MHC) molecule, and the conjugate is a polynucleotide-candidate peptide conjugate,

wherein the polynucleotide of each conjugate is attached to the candidate peptide of the conjugate and uniquely identifies the candidate peptide,

wherein in each complex, the candidate peptide of the conjugate binds to the MHC molecule and is between 5 and 40 amino acid residues in length, inclusive, and

wherein the candidate peptides in the plurality of complexes form a library of candidates for binding to a T-cell receptor (TCR).