US 11,913,949 B2
Lateral flow membrane designed for multiparameter readouts and compact multiparameter lateral flow immunoassay device comprising the same
Denise Van Rossum, Adelebsen (DE); Eric Jallérat, Ville d'Avray (FR); Susanne Drebing, Hardegsen (DE); and Dieter Melzner, Göttingen (DE)
Assigned to Sartorius Stedim Biotech GmbH, (DE)
Appl. No. 15/315,553
Filed by Sartorius Stedim Biotech GmbH, Göttingen (DE)
PCT Filed Apr. 28, 2015, PCT No. PCT/EP2015/000869
§ 371(c)(1), (2) Date Dec. 1, 2016,
PCT Pub. No. WO2015/188906, PCT Pub. Date Dec. 17, 2015.
Claims priority of application No. 14290166 (EP), filed on Jun. 10, 2014.
Prior Publication US 2018/0143191 A1, May 24, 2018
Int. Cl. G01N 33/543 (2006.01); G01N 33/76 (2006.01)
CPC G01N 33/54388 (2021.08) [G01N 33/76 (2013.01); B01L 2300/0883 (2013.01); G01N 2333/59 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A multiparameter lateral flow membrane for testing multiple ligands or analytes in a liquid sample, comprising a microporous membrane layer for lateral flow of a liquid through the microporous membrane layer,
wherein the microporous membrane layer has two or more flow lanes in the direction of lateral flow, each of the flow lanes constituting a single, unitary flow lane
wherein said two or more flow lanes run parallel and adjacent to one another and are separated by hydrophobic separation channels, where adjacent flow lanes are exclusively separated by a shared hydrophobic separation channel over the length of the adjacent flow lanes, and
wherein each of said two or more flow lanes comprises a detection zone including a binding agent,
wherein said two or more flow lanes are isomorphic lanes,
wherein said two or more flow lanes are zig-zag-shaped lanes or meander-shaped lanes; and
wherein the detection zone in a first flow lane, and the detection zone in a second flow lane adjacent to the first flow lane are positioned along a common line in the direction of fluid flow.