CPC G01N 33/5308 (2013.01) [C12N 9/0006 (2013.01); G01N 33/542 (2013.01); G01N 33/6812 (2013.01); C12Y 101/01049 (2013.01); G01N 2800/347 (2013.01)] | 17 Claims |
1. A method for conducting an assay for an analyte in a sample, comprising:
(a) conducting a sample first reaction sequence comprising:
i. forming a sample first reaction mixture by contacting the sample with an anti-analyte antibody, a conjugate comprising the analyte and an enzyme, and a substrate that produces a signal when in contact with the enzyme, and
ii. measuring signal from the sample first reaction mixture;
(b) conducting a sample second reaction sequence comprising,
i. forming a sample second reaction mixture by contacting the sample with the substrate and an anti-analyte antibody,
ii. measuring signal from the sample second reaction mixture;
(c) subtracting an amount of signal from step (b) from an amount of signal from step (a) to provide a net signal,
(d) using the net signal to determine the amount of the analyte in the sample.
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