CPC G01N 21/6408 (2013.01) [G01N 33/15 (2013.01)] | 8 Claims |
1. A determination method of a supramolecular organization of target luminescent molecules encapsulated within nanocarrier particles by a lifetime analysis comprising the steps of:
defining at least a first supramolecular organization of a plurality of target luminescent molecules and/or the nanocarrier particles, wherein the plurality of target luminescent molecules and/or the nanocarrier particles are substantially organized and optionally the nanocarrier particle is further luminescent;
receiving at least a first standard luminescence lifetime dataset representing a first decay associated to a first standard substance where the plurality of target luminescent molecules and/or the nanocarrier particles are substantially organized according to the first supramolecular organization;
processing a test substance comprising nanocarriers with the plurality of target luminescent molecules to obtain a test luminescence lifetime dataset of the test substance comprising a target luminescent molecule having an unknown supramolecular organization;
quantitatively comparing the test luminescence lifetime dataset and the at least first standard luminescence lifetime dataset;
determining whether a second supramolecular organization of the plurality of target luminescent molecules and the nanocarrier particles is present or not in the test substance based on a quantitative superimposition of the test luminescence lifetime dataset and the first standard luminescence lifetime dataset.
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