US 11,913,882 B2
Determination of the supramolecular organization of encapsulated molecules by luminescence lifetime analysis
Francesco Cardarelli, Pisa (IT); Fabio Beltram, Pisa (IT); Paolo Maria Tentori, Genoa (IT); Giulio Caracciolo, Rome (IT); and Daniela Pozzi, Rome (IT)
Assigned to SCUOLA NORMALE SUPERIORE, Pisa (IT); UNIVERSITÀ DEGLI STUDI DI ROMA “LA SAPIENZA”, Rome (IT); and FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA, Genoa (IT)
Appl. No. 18/252,202
Filed by SCUOLA NORMALE SUPERIORE, Pisa (IT); UNIVERSITÀ DEGLI STUDI DI ROMA “LA SAPIENZA”, Rome (IT); and FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA, Genoa (IT)
PCT Filed Nov. 8, 2021, PCT No. PCT/IB2021/060300
§ 371(c)(1), (2) Date May 9, 2023,
PCT Pub. No. WO2022/097108, PCT Pub. Date May 12, 2022.
Claims priority of application No. 102020000026699 (IT), filed on Nov. 9, 2020.
Prior Publication US 2023/0341326 A1, Oct. 26, 2023
Int. Cl. G01N 21/64 (2006.01); G01N 33/15 (2006.01)
CPC G01N 21/6408 (2013.01) [G01N 33/15 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A determination method of a supramolecular organization of target luminescent molecules encapsulated within nanocarrier particles by a lifetime analysis comprising the steps of:
defining at least a first supramolecular organization of a plurality of target luminescent molecules and/or the nanocarrier particles, wherein the plurality of target luminescent molecules and/or the nanocarrier particles are substantially organized and optionally the nanocarrier particle is further luminescent;
receiving at least a first standard luminescence lifetime dataset representing a first decay associated to a first standard substance where the plurality of target luminescent molecules and/or the nanocarrier particles are substantially organized according to the first supramolecular organization;
processing a test substance comprising nanocarriers with the plurality of target luminescent molecules to obtain a test luminescence lifetime dataset of the test substance comprising a target luminescent molecule having an unknown supramolecular organization;
quantitatively comparing the test luminescence lifetime dataset and the at least first standard luminescence lifetime dataset;
determining whether a second supramolecular organization of the plurality of target luminescent molecules and the nanocarrier particles is present or not in the test substance based on a quantitative superimposition of the test luminescence lifetime dataset and the first standard luminescence lifetime dataset.