Assay for detecting closely-related serotypes of human papillomavirus (HPV)
Chi Chen, Cockeysville, MD (US); Hugh J. Peck, Jarrettsville, MD (US); Michael Porter, Baltimore, MD (US); Gregory A. Richart, York, PA (US); and Ray A. McMillian, Timonium, MD (US)
Assigned to Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed by BECTON DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed on Nov. 11, 2020, as Appl. No. 17/094,908.
Application 17/094,908 is a division of application No. 15/843,957, filed on Dec. 15, 2017, granted, now 10,865,455.
Application 15/843,957 is a division of application No. 14/179,113, filed on Feb. 12, 2014, granted, now 9,868,997, issued on Jan. 16, 2018.
Application 14/179,113 is a division of application No. 13/028,589, filed on Feb. 16, 2011, granted, now 8,658,397, issued on Feb. 25, 2014.
Claims priority of provisional application 61/304,941, filed on Feb. 16, 2010.
Prior Publication US 2021/0071272 A1, Mar. 11, 2021
Int. Cl. C12Q 1/70 (2006.01)
CPC C12Q 1/708 (2013.01)
19 Claims
1. A probe set for the detection of multiple serotypes of human papillomavirus (HPV) comprising at least three oligonucleotide primer/probe sets, each of which comprises at least one oligonucleotide probe that is detectably labeled and has a nucleotide sequence length of about 10 to about 50 and at least two oligonucleotide primers each of which has a nucleotide sequence length of about 10 to about 150 wherein each primer/probe set is at least preferential for a specific serotype of the HPV organism wherein the first and second of the primer/probe sets are degenerate with respect to each other and wherein the degenerate probes each have a signal moiety that emits signal that is detectable on the same channel and wherein the third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype that is not the serotypes to which the degenerate primer/probe sets preferentially anneal and wherein the third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength of the probes in the first and second primer/probe sets, wherein the target for the degenerate primer/probe set is selected from the group consisting of SEQ ID NOS: 36-38.