CPC C12Q 1/6897 (2013.01) [C12N 9/1247 (2013.01); C12Q 1/48 (2013.01); C12Q 1/689 (2013.01); C12Y 207/07006 (2013.01)] | 13 Claims |
1. A system comprising:
a) a first polymerase domain attached to a first interaction component;
b) a second polymerase domain attached to a second interaction component;
c) a nucleic acid construct comprising a reporter gene under the control of a first heterologous promoter responsive to polymerase activity from the first and second polymerase domains when the first and second interaction components interact,
wherein the first polymerase domain comprises amino acids 1-179 of a T7 RNA polymerase and the second polymerase domain comprises amino acids 180-883 of a T7 RNA polymerase; wherein the system further comprises a negative control nucleic acid encoding a polypeptide that does not interact with the first or second interaction component; and wherein the polypeptide encoded by the negative control nucleic acid is attached to an alternate first polymerase domain comprising amino acids 1-179 of a T7 RNA polymerase or an alternate second polymerase domain comprising amino acids 180-883 of a T7 RNA polymerase and the system further comprises a second heterologous promoter responsive to polymerase activity from the interaction of the first and alternate second polymerase domains or the alternate first and the second polymerase domains.
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