CPC C12Q 1/6806 (2013.01) [C12Q 2600/112 (2013.01); C12Q 2600/158 (2013.01)] | 29 Claims |
1. A method comprising:
(a) providing a first mixture comprising a single-stranded ribonucleic acid (ssRNA) and a double-stranded deoxyribonucleic acid (ds-DNA);
(b) reverse transcribing the ssRNA in the first mixture, wherein the reverse transcribing comprises labeling the ssRNA with a first molecular barcode to produce a barcoded double- stranded complementary DNA (ds-cDNA), thereby obtaining a second mixture comprising the ds-DNA and the barcoded ds-cDNA; and
(c) bringing the second mixture or derivative thereof in contact with a plurality of adaptors comprising a second molecular barcode, to couple adaptors of the plurality of adaptors to the ds-DNA and the barcoded ds-cDNA.
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