US 11,913,053 B2
Application of trehalase in fermentative production
Jie Lin, Nanjing (CN); Hongxian Xu, Nanjing (CN); and Hui Peng, Nanjing (CN)
Assigned to Nanjing Bestzyme Bio-Engineering Co., Ltd., Jiangsu (CN)
Appl. No. 17/277,211
Filed by Nanjing Bestzyme Bio-Engineering Co., Ltd., Jiangsu (CN)
PCT Filed Sep. 17, 2019, PCT No. PCT/CN2019/106078
§ 371(c)(1), (2) Date Mar. 17, 2021,
PCT Pub. No. WO2020/057476, PCT Pub. Date Mar. 26, 2020.
Claims priority of application No. 201811083590.3 (CN), filed on Sep. 17, 2018.
Prior Publication US 2021/0403957 A1, Dec. 30, 2021
Int. Cl. C12P 7/06 (2006.01); C12P 13/08 (2006.01); C12P 13/14 (2006.01)
CPC C12P 7/06 (2013.01) [C12P 13/08 (2013.01); C12P 13/14 (2013.01); C12Y 302/01028 (2013.01)] 20 Claims
 
1. A method for producing a fermented product comprising an alcohol, wherein the method comprises:
(a) adding amylase to liquefy an alcohol fermentation raw material;
(b) saccharifying the liquefied raw material;
(c) adding yeast and performing fermentation;
(d) collecting alcohol mature mash supernatant after the end of fermentation; and
(e) adding a polypeptide with trehalase activity to the alcohol mature mash supernatant at an amount of 0.05-10 U/g DS to produce the fermented product, wherein the polypeptide comprises at least 95% sequence identity to the polypeptide of SEQ ID NO: 6.