Gjalt W. Huisman, Redwood City, CA (US); Nicholas J. Agard, San Francisco, CA (US); Benjamin Mijts, San Carlos, CA (US); Jonathan Vroom, South San Francisco, CA (US); and Xiyun Zhang, Fremont, CA (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on Sep. 2, 2020, as Appl. No. 17/009,925.
Application 17/009,925 is a division of application No. 16/653,061, filed on Oct. 15, 2019, granted, now 10,941,391.
Application 16/653,061 is a division of application No. 16/176,986, filed on Oct. 31, 2018, granted, now 10,494,623, issued on Dec. 3, 2019.
Application 16/176,986 is a division of application No. 15/431,491, filed on Feb. 13, 2017, granted, now 10,160,963, issued on Dec. 25, 2018.
Application 15/431,491 is a division of application No. 14/255,539, filed on Apr. 17, 2014, granted, now 9,611,468, issued on Apr. 4, 2017.
Claims priority of provisional application 61/897,932, filed on Oct. 31, 2013.
Claims priority of provisional application 61/813,586, filed on Apr. 18, 2013.
Prior Publication US 2020/0399627 A1, Dec. 24, 2020
1. An engineered polynucleotide encoding an engineered polypeptide comprising an amino acid sequence with at least 95% sequence identity to reference sequence SEQ ID NO:24, and wherein the engineered polypeptide comprises an amino acid residue difference at positions 158, 256, and 399, wherein the amino acid positions are numbered with reference to SEQ ID NO:24.