	US 11,913,040 B2
	Evolution of TALENs
David R. Liu, Cambridge, MA (US); Basil Hubbard, Cambridge, MA (US); and Ahmed Hussein Badran, Cambridge, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Jun. 23, 2021, as Appl. No. 17/355,735.
Application 17/355,735 is a continuation of application No. 16/804,228, filed on Feb. 28, 2020, granted, now 11,078,469.
Application 16/804,228 is a continuation of application No. 15/748,053, granted, now 10,612,011, previously published as PCT/US2016/044546, filed on Jul. 28, 2016.
Claims priority of provisional application 62/198,906, filed on Jul. 30, 2015.
Prior Publication US 2021/0403887 A1, Dec. 30, 2021
Int. Cl. C12N 9/22 (2006.01); C12N 15/62 (2006.01); C12N 15/85 (2006.01); C12N 15/90 (2006.01); A61K 48/00 (2006.01)

CPC C12N 9/22 (2013.01) [C12N 15/62 (2013.01); C12N 15/8509 (2013.01); C12N 15/90 (2013.01); A61K 48/0066 (2013.01); A61K 48/0091 (2013.01)]

20 Claims

1. A protein comprising an amino acid sequence that is at least 80% identical to the amino acid sequence LTPX₁QVVAIAX₂X₃X₄GGX₅X₆ALETVQRLLPVLCQX₇HG (SEQ ID NO: 2),

wherein X₁is D, E, or A, wherein X₂is S or N, wherein X₃is N or H, wherein X₄is G, D, I, or N, wherein X₅is K or R, wherein X₆is Q or P, and wherein X₇is D or A,

wherein the amino acid sequence comprises one or more amino acid substitutions selected from the group consisting of T2A, P3L, P3S, X₁4G, X₁4K, X₁4N, X₂11K, X₂11Y, X₃12H, X₄13K, X₄13H, G15S, X₅16R, X₆17P, T21A, L26F, P27S, V28G, Q31K, X₇₌S, D32E, and H33L, and

wherein the protein binds to a target sequence of a DNA molecule.