	US 11,913,035 B2
	Modified seed oil content by gene editing
Kayla S Flyckt, Ankeny, IA (US); Kristin Haug Collet, Des Moines, IA (US); Zhan-Bin Liu, Clive, IA (US); Keith R Roesler, Urbandale, IA (US); Bo Shen, Johnston, IA (US); and Laura L Wayne, Des Moines, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., , IA (US)
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed on Sep. 19, 2022, as Appl. No. 17/933,358.
Application 17/933,358 is a continuation of application No. 17/057,056, granted, now 11,479,759, previously published as PCT/US2019/034606, filed on May 30, 2019.
Claims priority of provisional application 62/796,847, filed on Jan. 25, 2019.
Claims priority of provisional application 62/679,116, filed on Jun. 1, 2018.
Prior Publication US 2023/0151338 A1, May 18, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/10 (2006.01); C12N 15/82 (2006.01)

CPC C12N 9/1029 (2013.01) [C12N 15/8213 (2013.01); C12N 15/8247 (2013.01); C12Y 203/0102 (2013.01)]

20 Claims

1. A modified polynucleotide encoding a diacylglycerol acyltransferase-1 (DGAT1) polypeptide having at least 95% identity to SEQ ID NO:2, the polynucleotide encoding a modification comprising a non-asparagine at the position corresponding to position 473 of SEQ ID NO:2, wherein when expressed in a plant cell, the polynucleotide increases the fatty acid content of the plant cell compared to a plant cell comprising a comparable polynucleotide without the modification.