US 11,913,029 B2
Platforms and systems for automated cell culture
Matthias Wagner, Cambridge, MA (US); Suvi Aivio, Arlington, MA (US); Mariangela Amenduni, Arlington, MA (US); Catherine Pilsmaker, Arlington, MA (US); Arnaldo Pereira, Cambridge, MA (US); Ananya Zutshi, Boston, MA (US); Anthia Toure, Boston, MA (US); Steven Nagle, Wayland, MA (US); Ozge Whiting, Pawtucket, RI (US); George Harb, Providence, RI (US); Matthew Sullivan, Westwood, MA (US); Maya Berlin-Udi, Acton, MA (US); Stefanie Morgan, Hanover, MA (US); Nick Seay, Charlottesville, VA (US); Sang Lee, Newton, MA (US); and Scott Luro, Somerville, MA (US)
Assigned to CELLINO BIOTECH, INC., Cambridge, MA (US)
Filed by Cellino Biotech, Inc., Cambridge, MA (US)
Filed on Dec. 5, 2022, as Appl. No. 18/061,811.
Application 18/061,811 is a continuation of application No. 17/688,854, filed on Mar. 7, 2022.
Claims priority of provisional application 63/216,558, filed on Jun. 30, 2021.
Claims priority of provisional application 63/249,698, filed on Sep. 29, 2021.
Claims priority of provisional application 63/288,859, filed on Dec. 13, 2021.
Claims priority of provisional application 63/167,114, filed on Mar. 28, 2021.
Claims priority of provisional application 63/222,059, filed on Jul. 15, 2021.
Claims priority of provisional application 63/239,995, filed on Sep. 2, 2021.
Claims priority of provisional application 63/282,351, filed on Nov. 23, 2021.
Claims priority of provisional application 63/295,968, filed on Jan. 3, 2022.
Claims priority of provisional application 63/298,241, filed on Jan. 11, 2022.
Claims priority of provisional application 63/210,243, filed on Jun. 14, 2021.
Claims priority of provisional application 63/157,731, filed on Mar. 7, 2021.
Claims priority of provisional application 63/297,290, filed on Jan. 7, 2022.
Claims priority of provisional application 63/194,306, filed on May 28, 2021.
Claims priority of provisional application 63/284,839, filed on Dec. 1, 2021.
Claims priority of provisional application 63/226,128, filed on Jul. 27, 2021.
Claims priority of provisional application 63/311,673, filed on Feb. 18, 2022.
Claims priority of provisional application 63/196,904, filed on Jun. 4, 2021.
Prior Publication US 2023/0095664 A1, Mar. 30, 2023
Int. Cl. G06T 7/00 (2017.01); B01L 3/00 (2006.01); B01L 9/00 (2006.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12M 3/00 (2006.01); C12N 5/00 (2006.01); C12N 5/074 (2010.01); G01N 15/10 (2006.01); G01N 15/14 (2006.01)
CPC C12N 5/0696 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); B01L 9/52 (2013.01); C12M 23/14 (2013.01); C12M 23/22 (2013.01); C12M 23/26 (2013.01); C12M 23/28 (2013.01); C12M 23/42 (2013.01); C12M 23/44 (2013.01); C12M 23/48 (2013.01); C12M 25/06 (2013.01); C12M 27/02 (2013.01); C12M 29/02 (2013.01); C12M 31/02 (2013.01); C12M 33/00 (2013.01); C12M 33/12 (2013.01); C12M 41/12 (2013.01); C12M 41/26 (2013.01); C12M 41/44 (2013.01); C12M 41/46 (2013.01); C12M 41/48 (2013.01); C12M 47/04 (2013.01); C12N 5/0081 (2013.01); G01N 15/1056 (2013.01); G01N 15/1429 (2013.01); G01N 15/1434 (2013.01); G01N 15/1475 (2013.01); G06T 7/0012 (2013.01); G06T 7/0016 (2013.01); B01L 2200/18 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0877 (2013.01); C12M 41/36 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1452 (2013.01); G01N 2015/1454 (2013.01); G01N 2015/1486 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/20081 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A modular bioprocessing system, comprising:
(a) one or more cell culture process modules, wherein each of the one or more cell culture process modules is configured to:
(i) receive one or more cell culture containers, each of the one or more cell culture containers configured for performing one or more cell culture processes therein, wherein each of the one or more cell culture containers comprises at least one semi-transparent surface permanently attached thereto, wherein the at least one semi-transparent surface is configured to enable light-based cell imaging when illuminated with light within a first wavelength range and enable light-based cell manipulation when illuminated with light within a second wavelength range; and
(ii) manipulate the one or more cell culture processes being performed within the one or more cell culture containers, wherein manipulating the one or more cell culture processes comprises performing the light-based cell manipulation, wherein the light-based cell manipulation comprises directing the light within the second wavelength range toward adherent cells in the one or more cell culture containers, such that the directed light within the second wavelength range is at least partially absorbed by the at least one semi-transparent surface, to selectively lyse the adherent cells in the one or more cell culture containers; and
(b) a computing subsystem communicatively coupled to the one or more cell culture process modules, the computing subsystem configured to:
(i) process images of the one or more cell culture processes; and
(ii) manage the one or more cell culture processes being performed within the one or more cell culture containers based at least in part on the image processing, wherein managing the one or more cell culture processes comprises controlling the one or more cell culture process modules to manipulate the one or more cell culture processes.