	US 11,913,005 B2
	Optimized CRISPR-Cas nucleases and base editors and methods of use thereof
Nathaniel Graham, Durham, NC (US)
Assigned to Pairwise Plants Services, Inc., Durham, NC (US)
Filed by Pairwise Plants Services, Inc., Durham, NC (US)
Filed on Oct. 20, 2022, as Appl. No. 18/048,236.
Application 18/048,236 is a division of application No. 17/078,576, filed on Oct. 23, 2020, granted, now 11,591,607.
Claims priority of provisional application 62/925,422, filed on Oct. 24, 2019.
Prior Publication US 2023/0114342 A1, Apr. 13, 2023
Int. Cl. C12N 15/82 (2006.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01)

CPC C12N 15/8218 (2013.01) [C12N 9/22 (2013.01); C12N 15/113 (2013.01); C12N 2310/20 (2017.05)]

8 Claims

1. A method of increasing the stability of a base editor in a prokaryotic cell, the method comprising:

(a) providing a base editor comprising a deaminase domain and a CRISPR-Cas nuclease operably associated with a promoter region, wherein the promoter region comprises an intron; and

(b) introducing the base editor into a prokaryote, wherein the number of mutations in the base editor is reduced when compared to an identical base editor comprising a promoter region that does not comprise an intron, thereby increasing the stability of the base editor.