US 11,912,990 B2
Systems and methods for biomolecule retention
Tural Aksel, Redwood City, CA (US); Stephen Hendricks, Los Gatos, CA (US); Elvis Ikwa, San Leandro, CA (US); Pierre Indermuhle, Berkeley, CA (US); Sadie Ingle, Suttons Bay, MI (US); Christina Inman, San Mateo, CA (US); Parag Mallick, San Mateo, CA (US); Torri Elise Rinker, San Francisco, CA (US); and Steven Tan, San Mateo, CA (US)
Assigned to NAUTILUS SUBSIDIARY, INC., Seattle, WA (US)
Filed by Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed on Jul. 28, 2023, as Appl. No. 18/361,731.
Application 18/361,731 is a continuation of application No. 18/050,732, filed on Oct. 28, 2022, granted, now 11,760,997.
Application 18/050,732 is a continuation of application No. 17/692,035, filed on Mar. 10, 2022, granted, now 11,505,796, issued on Nov. 22, 2022.
Claims priority of provisional application 63/256,761, filed on Oct. 18, 2021.
Claims priority of provisional application 63/159,500, filed on Mar. 11, 2021.
Prior Publication US 2023/0407294 A1, Dec. 21, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6804 (2018.01); B01J 19/00 (2006.01); B82Y 5/00 (2011.01); C12Q 1/6837 (2018.01)
CPC C12N 15/1093 (2013.01) [B01J 19/0046 (2013.01); B82Y 5/00 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6837 (2013.01); B01J 2219/0061 (2013.01)] 29 Claims
 
1. A method, comprising:
a) providing a plurality of nucleic acid nanostructures, wherein each nucleic acid nanostructure is coupled to one and only one polypeptide by a separating group, wherein the separating group provides an average separation distance of at least 10 nanometers between the one and only one polypeptide and a nucleic acid nanostructure to which the one and only polypeptide is coupled;
b) coupling the plurality of nucleic acid nanostructures to a plurality of sites of an array, wherein the plurality of nucleic acid nanostructures comprises a first polypeptide species and a second polypeptide species having a dynamic range of at least 104; and
c) detecting presence of the first polypeptide species and the second polypeptide species at individual sites of the array.