	US 11,912,983 B2
	Polyanionic acids to improve recovery and minimize system loss
Michael Donegan, Charlton, MA (US); Martin Gilar, Franklin, MA (US); Matthew Lauber, North Smithfield, RI (US); Scott Mccall, Smithfield, RI (US); Pamela Iraneta, Brighton, MA (US); and Kerri Smith, Marlborough, MA (US)
Assigned to Waters Technologies Corporation, Milford, MA (US)
Filed by Waters Technologies Corporation, Milford, MA (US)
Filed on Dec. 4, 2020, as Appl. No. 17/112,502.
Claims priority of provisional application 62/944,145, filed on Dec. 5, 2019.
Prior Publication US 2021/0171932 A1, Jun. 10, 2021
Int. Cl. C12N 15/10 (2006.01); C12N 15/113 (2010.01)

CPC C12N 15/101 (2013.01) [C12N 15/113 (2013.01); C12N 2310/11 (2013.01); C12N 2310/315 (2013.01)]

15 Claims

1. A method of performing solid phase extraction comprising:

injecting a sample comprising oligonucleotides into a solid phase extraction cartridge comprising a stationary phase, wherein the oligonucleotides are retained by the stationary phase;

washing the solid phase extraction cartridge with a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M to elute the oligonucleotides from the solid phase extraction cartridge.