US 11,912,823 B2
Methods of preparing a poloxamer for use in cell culture medium
Ganesh Prasadh Vissvesvaran, South San Francisco, CA (US); Robert David Kiss, South San Francisco, CA (US); Steven J. Meier, South San Francisco, CA (US); Inchan Kwon, Charlottesville, VA (US); Kara Calhoun, South San Francisco, CA (US); Kate Winchester, South San Francisco, CA (US); Amelia Adams, South San Francisco, CA (US); Marion Glenn, South San Francisco, CA (US); Stefan Koenig, South San Francisco, CA (US); and Alan Deese, South San Francisco, CA (US)
Assigned to Genentech, Inc., South San Francisco, CA (US)
Filed by Genentech, Inc., South San Francisco, CA (US)
Filed on May 13, 2021, as Appl. No. 17/319,886.
Application 17/319,886 is a division of application No. 15/273,155, filed on Sep. 22, 2016, granted, now 11,034,793.
Application 15/273,155 is a continuation of application No. PCT/US2015/022592, filed on Mar. 25, 2015.
Claims priority of provisional application 61/970,281, filed on Mar. 25, 2014.
Prior Publication US 2021/0371587 A1, Dec. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C08G 65/30 (2006.01); C12N 5/00 (2006.01); C07K 16/00 (2006.01)
CPC C08G 65/30 (2013.01) [C07K 16/00 (2013.01); C12N 5/0018 (2013.01); C07K 2317/14 (2013.01); C08G 2650/50 (2013.01); C12N 2500/50 (2013.01)] 13 Claims
 
1. A method of producing a polypeptide in a cell culture, comprising the step of culturing a cell that produces the polypeptide in a cell culture medium under conditions suitable for production of the polypeptide, wherein the cell culture medium comprises a poloxamer prepared by a method comprising:
(a) heating a solid poloxamer to form a liquid poloxamer, wherein the solid poloxamer is heated to:
(1) between 157° C. and 185° C. for at least 1 minute,
(2) between 134° C. and 157° C. for at least 1 minute,
(3) between 120° C. and 134° C. for at least 62 minutes,
(4) between 100° C. and 120° C. for at least 98 minutes,
(5) between 80° C. and 100° C. for at least 122 minutes, or
(6) between 60° C. and 80° C. for at least 143 minutes; and
(b) cooling the liquid poloxamer to a temperature below 50° C. to form a solid heat-treated poloxamer, wherein the cooling is not conducted in a prilling or milling device, wherein the poloxamer comprises a copolymer of ethylene oxide and propylene oxide; and
wherein the poloxamer comprises a copolymer having a formula of HO(C2H4O)n(C3H6O)m(C2H4O)nH with n having a value of 80, with m having a value of 27, and the poloxamer has an average molecular weight of from 7680 to 9510 g/mol.