US 11,912,696 B2
Compounds and methods for detection of nitric oxide
Matthew A. Larsen, Madison, WI (US); Hui Wang, Madison, WI (US); Wenhui Zhou, Madison, WI (US); Peter Hofsteen, Madison, WI (US); and Jolanta Vidugiriene, Madison, WI (US)
Assigned to Promega Corporation, Madison, WI (US)
Filed by Promega Corporation, Madison, WI (US)
Filed on Sep. 30, 2022, as Appl. No. 17/937,210.
Claims priority of provisional application 63/251,395, filed on Oct. 1, 2021.
Prior Publication US 2023/0117418 A1, Apr. 20, 2023
Int. Cl. C07D 417/04 (2006.01); C07D 277/64 (2006.01)
CPC C07D 417/04 (2013.01) [C07D 277/64 (2013.01)] 20 Claims
 
1. A compound of formula (I):

OG Complex Work Unit Chemistry
or a salt thereof, wherein:
R1 is selected from —CN and

OG Complex Work Unit Chemistry
R2 is selected from hydrogen and halo;
R3 is selected from hydrogen, C1-C6 alkyl, and hydroxy-C1-C6-alkyl;
n is 0, 1, 2, or 3;
each R4 is independently selected from C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C1-C6 heteroalkyl, C1-C6 alkoxy, C1-C6 haloalkyl, C1-C6 haloalkoxy, halo, hydroxy, amino, cyano, nitro, —COORa, and —CONRbRc;
R5 is selected from hydrogen and C1-C4 alkyl; and
Ra, Rb, and Rc are each independently selected from hydrogen, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, and C1-C6 heteroalkyl.