US 11,912,599 B2
Enzymatic purification of water
Martin Ryen, Uppsala (SE)
Assigned to Pharem Biotech AB, Uppsala (SE)
Appl. No. 16/486,067
Filed by Pharem Biotech AB, Uppsala (SE)
PCT Filed Feb. 14, 2018, PCT No. PCT/SE2018/050137
§ 371(c)(1), (2) Date Aug. 14, 2019,
PCT Pub. No. WO2018/151648, PCT Pub. Date Aug. 23, 2018.
Claims priority of application No. 1750142-0 (SE), filed on Feb. 15, 2017.
Prior Publication US 2020/0189947 A1, Jun. 18, 2020
Int. Cl. C02F 3/34 (2023.01); C02F 3/10 (2023.01); C02F 101/30 (2006.01); C02F 103/34 (2006.01)
CPC C02F 3/342 (2013.01) [C02F 3/108 (2013.01); C02F 3/348 (2013.01); C02F 2101/305 (2013.01); C02F 2101/306 (2013.01); C02F 2103/343 (2013.01); C02F 2203/006 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A filter for purifying sewage water flowing through said filter from a plurality of environmentally hazardous organic substances, said filter comprising:
a H2O stable open-cell foam material, and
a plurality of enzymes covalently bound to the H2O stable open-cell foam material, wherein said plurality of enzymes have the ability to eliminate said plurality of environmentally hazardous organic substances upon contact with said sewage water comprising said plurality of environmentally hazardous organic substances chosen from drug molecules in the group of ciprofloxacin, citalopram, clarithromycin, diclofenac, erythromycin, estradiol, etinylestradiol, fluconazole, ibuprofen, carbamazepine, ketoconazole, levonorgestrel, losartan, metoprolol, methotrexate, naproxen, oxazepam, sertraline, sulfametoxazole, tramadol, trimetroprim, zolpidem, ketoprofen, risperidone, fluoxetine, venlafaxine, ofloxacin, doxycycline, norfloxacin and tetracycline, and
wherein said plurality of enzymes will eliminate said plurality of environmentally hazardous organic substances present in said sewage water,
wherein the plurality of enzymes are chosen from a group consisting of Epoxide hydrolase, P450 BM3, Melamine Deaminase, Acetoacetate decarboxylase, and LinB, and
wherein the plurality of enzymes have the ability, or are modified through mutagenesis to possess the ability, to eliminate the plurality of environmentally hazardous organic substance(s) at a pH in a range of 5.5 to 8.5 and a temperature in a range of +8° C. to 20° C., while maintaining an activity against the environmentally hazardous organic substances of at least 103 s−1M−1 (kcat/Km) and
said filter having the ability to purify the sewage water from said drug molecules to an extent of 60-95%.