	US 11,911,770 B2
	Point of care (POC) device for facilitating nucleic acid based testing and method thereof
Nandita Kedia, West Bengal (IN); Sujay Kumar Biswas, West Bengal (IN); Saptarshi Banerjee, West Bengal (IN); Aditya Bandopadhyay, West Bengal (IN); Arindam Mondal, West Bengal (IN); and Suman Chakraborty, West Bengal (IN)
Assigned to INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR, West Bengal (IN)
Filed by INDIAN INSTITUTE OF TECHNOLOGY KHARAGPUR, West Bengal (IN)
Filed on Aug. 2, 2022, as Appl. No. 17/816,908.
Application 17/816,908 is a division of application No. 17/201,968, filed on Mar. 15, 2021, granted, now 11,440,014.
Claims priority of application No. 202031031597 (IN), filed on Jul. 23, 2020.
Prior Publication US 2023/0182142 A1, Jun. 15, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6832 (2018.01); C12Q 1/686 (2018.01); B01L 7/00 (2006.01); B01L 3/00 (2006.01); C12Q 1/70 (2006.01); G01N 33/543 (2006.01)

CPC B01L 7/52 (2013.01) [B01L 3/502715 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6832 (2013.01); C12Q 1/701 (2013.01); G01N 33/54386 (2013.01); B01L 2300/126 (2013.01); B01L 2300/1805 (2013.01); B01L 2300/1844 (2013.01); G01N 2333/165 (2013.01)]

1 Claim

1. A set of primers suitable for desired SARS-CoV-2 detection with enhanced specificity and sensitivity wherein the set is selected from each of the primer sets directed to each gene selected from ORF 1b, N gene, E gene and RNaseP as hereunder:


Target	Primer
Region	Set	Sequence (5′ to 3′)

ORF1b	F3	GCCATTAGTGCAAAGAATAGAGC
	SEQ.
	ID
	No. 1

	B3	GGCATGGCTCTATCACATTTAGG
	SEQ.
	ID
	No. 2

	FIP	5′[Btn]TAGCTCCTCTAGTGGCGGCTATTGCACC
	(F1c +	GTAGCTGGTGTCTC
	F2)
	SEQ.
	ID
	No. 3

	BIP	TGTAGTAATTGGAACAAGCAAATTCTATGGTGGCC
	(B1c +	AACCCATAAGGTGAGGG
	B2)
	SEQ.
	ID
	No. 4

	Loop	TTTTTGATGAAACTGTCTATTGGTCATAGTACTAC
	F	AG
	SEQ.
	ID
	No. 5

	Loop	GGCACAACATGTTAAAAACTGTTTATAGTGATGTA
	B	G
	SEQ.
	ID
	No. 6

	BLP	5′[6FAM]TTGGCACAACATGTTAAAAACTGTTTA
	Probe	TAGTGATG
	SEQ.
	ID
	No. 7

	BLP	5′[6FAM]TTGGCACAACATGTTAAAAACTGTTTA
	3′-	TAGTGATG 3′-[3dG]
	mod
	Probe
	SEQ.
	ID
	No. 8

	FLP	5′-
	Probe	[6FAM]GCGGCTATTGATTTCAATAATTTTTGATG
	SEQ.	AAAC
	ID
	No. 9

N	F3	ACAATGTAACACAAGCTTTCG
gene	SEQ.
	ID
	No. 10

	B3	TTGGATCTTTGTCATCCAATT
	SEQ.
	ID
	No. 11

	FIP	5′[Btn]GGCCAATGTTTGTAATCAGTTCCTTAGA
	(F1c +	CGTGGTC CAGAACAA
	F2)
	SEQ.
	ID
	No. 12

	BIP	GCTTCAGCGTTCTTCGGAATCACCTGTGTAGGTCA
	(B1c +	ACC
	B2)
	SEQ.
	ID
	No. 13

	Loop	TGGTCCCCAAAATTTCCTTGG
	F
	SEQ.
	ID
	No. 14

	Loop	CGCGCATTGGCATGGAAGT
	B
	SEQ.
	ID
	No. 15

	BLP	5′-[6FAM] TTGGCATGGAAGTCACACCTTC
	Probe
	SEQ.
	ID
	No. 16

	BLP	5′-[6FAM] TTGGCATGGAAGTCACACCTTC
	3′-	3′-[3dC]
	mod
	Probe
	SEQ.
	ID
	No. 17

	FLP	5′-[6FAM] GATTAGTTCCTGGTCCCCAAAATTT
	Probe	CC
	SEQ.
	ID
	No. 18

E	F3	TTGTAAGCACAAGCTGATG
gene	SEQ.
	ID
	No. 19

	B3	AGAGTAAACGTAAAAAGAAGGTT
	SEQ.
	ID
	No. 20

	FIP	5′[Btn]CGAAAGCAAGAAAAAGAAGTACGCTAGT
	(F1c +	ACGAACTTATGTACTCATTCG
	F2)
	SEQ.
	ID
	No. 21

	BIP	GGTATTCTTGCTAGTTACACTAGCCAAGACTCACG
	(B1c +	TTAACAATATTGC
	B2)
	SEQ.
	ID
	No. 22

	Loop	ATTAACGTACCTGTCTCTTCCGAAA
	F
	SEQ.
	ID
	No. 23

	Loop	ATCCTTACTGCGCTTCGATTGTGTG
	B
	SEQ.
	ID
	No. 24

	BLP	5′-[6FAM] ATCCTTACTGCGCTTCGATTGTGTG
	Probe
	SEQ.
	ID
	No. 25

	BLP	5′-[6FAM] ATCCTTACTGCGCTTCGATTGTGTG
	3′-	3′-[3dG]
	mod
	Probe
	SEQ.
	ID
	No. 26

	FLP	5′-[6FAM] ATTAACTATTAACGTACCTGTCTCT
	Probe	TCC
	SEQ.
	ID
	No. 27

RNaseP	F3	TTGATGAGCTGGAGCCA
	SEQ.
	ID
	No. 28

	B3	CACCCTCAATGCAGAGTC
	SEQ.
	ID
	No. 29

	FIP	5′[Btn]GTGTGACCCTGAAGACTCGGTTTTAGCC
	(F1c +	ACTGACTCGGATC
	F2)
	SEQ.
	ID
	No. 30

	BIP	CCTCCGTGATATGGCTCTTCGTTTTTTTCTTACAT
	(B1c +	GGCTCTGGTC
	B2)
	SEQ.
	ID
	No. 31

	Loop	ATGTGGATGGCTGAGTTGTT
	F
	SEQ.
	ID
	No. 32

	Loop	CATGCTGAGTACTGGACCTC
	B
	SEQ.
	ID
	No. 33

	BLP	5′-[6FAM] CATGCTGAGTACTGGACCTCG
	Probe
	SEQ.
	ID
	No. 34

	FLP	5′-[6FAM] ATGTGGATGGCTGAGTTGTT
	Probe
	SEQ.
	ID
	No. 35