CPC B01L 3/527 (2013.01) [C12Q 1/6869 (2013.01); G06T 7/0012 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0877 (2013.01); G06T 2207/30024 (2013.01)] | 20 Claims |
1. A method, comprising:
flowing an incorporation reagent, through a reagent management system and a flow cell of an instrument, the flow cell having a first polynucleotide positioned therein, wherein the incorporation reagent adds a first base onto a sequence of bases, the sequence of bases comprising a second polynucleotide complementary to the first polynucleotide;
capturing an image, of an identification signal emanating from the first base, after the first base has been added onto the second polynucleotide;
flowing a cleavage reagent, through the reagent management system and flow cell, to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide; and
flowing a buffer reagent, through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions to remove the cleavage reagent from the flow cell.
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