US 11,911,765 B2
Reagent exchange in an instrument
Tony Yen, San Diego, CA (US); Eric Stava, San Diego, CA (US); and Rajagopal Panchapakesan, Escondido, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Jun. 16, 2020, as Appl. No. 16/902,826.
Claims priority of provisional application 62/863,444, filed on Jun. 19, 2019.
Prior Publication US 2020/0398278 A1, Dec. 24, 2020
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6869 (2018.01); G06T 7/00 (2017.01)
CPC B01L 3/527 (2013.01) [C12Q 1/6869 (2013.01); G06T 7/0012 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0877 (2013.01); G06T 2207/30024 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method, comprising:
flowing an incorporation reagent, through a reagent management system and a flow cell of an instrument, the flow cell having a first polynucleotide positioned therein, wherein the incorporation reagent adds a first base onto a sequence of bases, the sequence of bases comprising a second polynucleotide complementary to the first polynucleotide;
capturing an image, of an identification signal emanating from the first base, after the first base has been added onto the second polynucleotide;
flowing a cleavage reagent, through the reagent management system and flow cell, to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide; and
flowing a buffer reagent, through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions to remove the cleavage reagent from the flow cell.