US 11,911,741 B2
Diatomaceous earth composition with a low endotoxin content
Karl Pflanz, Gleichen (DE); Florian Hebenstreit, Beberstedt (DE); and Andreas Pickl, Klein Schneed (DE)
Assigned to Sartorius Stedim Biotech GMBH, Gottingen (DE)
Appl. No. 16/648,521
Filed by Sartorius Stedim Biotech GMBH, Göttingen (DE)
PCT Filed Oct. 9, 2018, PCT No. PCT/EP2018/077456
§ 371(c)(1), (2) Date Mar. 18, 2020,
PCT Pub. No. WO2019/076683, PCT Pub. Date Apr. 25, 2019.
Claims priority of application No. 17001714 (EP), filed on Oct. 17, 2017.
Prior Publication US 2020/0261883 A1, Aug. 20, 2020
Int. Cl. B01J 20/14 (2006.01); B01J 20/30 (2006.01); B01J 20/28 (2006.01); B01D 37/02 (2006.01); A61L 2/08 (2006.01); C12M 1/00 (2006.01); B65D 65/40 (2006.01); B65B 55/02 (2006.01)
CPC B01J 20/14 (2013.01) [A61L 2/081 (2013.01); B01D 37/02 (2013.01); B01J 20/28004 (2013.01); B01J 20/2805 (2013.01); B01J 20/28016 (2013.01); B01J 20/3028 (2013.01); B65B 55/02 (2013.01); B65D 65/40 (2013.01); C12M 47/02 (2013.01); C12M 47/10 (2013.01); A61L 2202/21 (2013.01)] 7 Claims
 
1. A method for producing a diatomaceous earth composition with a low endotoxin content, comprising the following steps:
(a) providing calcined diatomaceous earth particles;
(b) mixing the calcined diatomaceous earth particles with water in a mass ratio of 1:1.0 to 1:2.0, thereby obtaining an agglomerated mixture of calcined diatomaceous earth particles and water;
(c) sealing the agglomerated mixture of calcined diatomaceous earth particles and water obtained in step (b) in a water-impermeable and γ-sterilizable packaging, thereby obtaining a sealed agglomerated mixture of calcined diatomaceous earth particles and water;
(d) sterilizing the sealed agglomerated mixture of calcined diatomaceous earth particles and water obtained in step (c) by exposure to γ-radiation, thereby obtaining a diatomaceous earth composition with an endotoxin content of equal to or less than 0.5 EU/mL; and
(e) optionally removing the packaging from the diatomaceous earth composition obtained in step (d).