US 11,911,482 B2
Self assembling protein nanoparticles as carrier molecules
Todd Yeates, Agoura Hills, CA (US); Yen-Ting Lai, Allston, MA (US); Justin Miller, Los Angeles, CA (US); Phillip Nguyen, Ithaca, NY (US); Yashes Srinivasan, San Diego, CA (US); Nithin Dharmaraj, San Diego, CA (US); Scott Taylor, Los Angeles, CA (US); and Andrew Liu, Monrovia, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Aug. 25, 2020, as Appl. No. 17/002,571.
Prior Publication US 2022/0062430 A1, Mar. 3, 2022
Int. Cl. A61K 47/62 (2017.01); A61K 47/65 (2017.01); A61K 47/69 (2017.01)
CPC A61K 47/62 (2017.08) [A61K 47/65 (2017.08); A61K 47/6929 (2017.08)] 15 Claims
 
1. A polypeptide comprising (a) a scaffolding protein consisting of an amino acid sequence having 100% sequence identity to SEQ ID NO: 64, (b) a passenger peptide covalently linked thereto, wherein (i) the passenger peptide is a peptide tag, a covalent peptide tag, a protein tag, an enzyme, or an enzyme substrate, and the passenger peptide neither comprises nor consists of SEQ ID NO: 41 or SEQ ID NO: 42, and/or (ii) the passenger peptide is covalently linked to an amino acid selected from the group consisting of: residues 19-26, 131-141, 245-257, 300-304, and 350-357 of SEQ ID NO: 64 or replaces one or more amino acids selected from the group consisting of: residues 19-26, 131-141, 245-257, 300-304, and 350-357 of SEQ ID NO: 64; and (c) optionally, a peptide having a sequence selected from the group consisting of SEQ ID NO: 45, SEQ ID NO: 52, and SEQ ID NO: 56 covalently attached to a terminal end of the scaffolding protein.