US 11,911,446 B2
Use of C-type natriuretic peptide variants to treat skeletal dysplasia
Sherry Bullens, Novato, CA (US); Stuart Bunting, Novato, CA (US); Tianwei Chou, Novato, CA (US); Augustus O. Okhamafe, Concord, CA (US); Christopher P. Price, Aachen (DE); Daniel J. Wendt, Novato, CA (US); and Clarence Yap, Novato, CA (US)
Assigned to BIOMARIN PHARMACEUTICAL INC., Novato, CA (US)
Filed by BioMarin Pharmaceutical Inc., Novato, CA (US)
Filed on Apr. 1, 2020, as Appl. No. 16/837,905.
Application 16/837,905 is a division of application No. 15/880,002, filed on Jan. 25, 2018, granted, now 10,646,550.
Application 15/880,002 is a division of application No. 15/225,355, filed on Aug. 1, 2016, granted, now 9,907,834.
Claims priority of provisional application 62/320,704, filed on Apr. 11, 2016.
Claims priority of provisional application 62/199,081, filed on Jul. 30, 2015.
Prior Publication US 2020/0246433 A1, Aug. 6, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/22 (2006.01); A61K 38/17 (2006.01); A61K 9/19 (2006.01); A61K 47/12 (2006.01); A61K 47/26 (2006.01); A61K 47/10 (2017.01); A61K 9/00 (2006.01); A61K 47/20 (2006.01); A61K 47/18 (2017.01)
CPC A61K 38/2242 (2013.01) [A61K 9/0019 (2013.01); A61K 9/19 (2013.01); A61K 38/1709 (2013.01); A61K 47/10 (2013.01); A61K 47/12 (2013.01); A61K 47/183 (2013.01); A61K 47/20 (2013.01); A61K 47/26 (2013.01)] 27 Claims
 
1. A method of increasing long bone growth in a subject in need thereof, said method comprising the step of administering to said subject a composition comprising:
(a) a CNP variant peptide in an amount of at least 7.5 μg/kg, wherein the CNP variant peptide is selected from the group consisting of:
 
 (CNP-37(M32N); SEQ ID NO: 1)
 
 QEHPNARKYKGANKKGLSKGCFGLKLDRIGSNSGLGC;
 
 
 
 (Met-CNP-37; SEQ ID NO: 2)
 
 MQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC;
 
 
 
 (Pro-CNP-37; SEQ ID NO: 3)
 
 PQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC;
 
 
 
 (Gly-CNP 37 (M32N); SEQ ID NO: 4)
 
 GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSNSGLGC;
 
 
 
 (Pro-Gly-CNP-37; SEQ ID NO: 5)
 
 PGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC;
 
 
 
 (Met-Gly-CNP-37; SEQ ID NO: 6)
 
 MGQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC;
 
 and
 
 
 
 (Gly-CNP-37 or CNP-28: SEQ ID NO: 7)
 
 GQEHPNARKYKGANKKGLSKGCFGLKLDRIGSMSGLGC,
 and
(b) citric acid monohydrate, sodium citrate dihydrate, trehalose dihydrate, D-mannitol, L-methionine and polysorbate 80,
wherein said step of administering increases long bone growth in said subject.