US 11,910,788 B2
Mouse having a humanized cluster of differentiation 47 gene
Cagan Gurer, Chappaqua, NY (US); Ella Ioffe, Bronx, NY (US); Alexander Mujica, Elmsford, NY (US); and Gavin Thurston, Briarcliff Manor, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Jan. 29, 2021, as Appl. No. 17/161,801.
Application 17/161,801 is a continuation of application No. 15/982,174, filed on May 17, 2018, granted, now 10,939,673.
Application 15/982,174 is a continuation of application No. 14/951,825, filed on Nov. 25, 2015, abandoned.
Claims priority of provisional application 62/087,992, filed on Dec. 5, 2014.
Prior Publication US 2021/0161112 A1, Jun. 3, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/027 (2006.01); C12N 15/85 (2006.01); G01N 33/50 (2006.01); C07K 16/28 (2006.01); C07K 14/705 (2006.01); C07K 14/47 (2006.01)
CPC A01K 67/0278 (2013.01) [C07K 14/4703 (2013.01); C07K 14/705 (2013.01); C07K 14/70503 (2013.01); C07K 14/70596 (2013.01); C07K 16/2803 (2013.01); C12N 15/8509 (2013.01); G01N 33/5011 (2013.01); G01N 33/5014 (2013.01); G01N 33/5055 (2013.01); G01N 33/5088 (2013.01); A01K 2207/12 (2013.01); A01K 2207/15 (2013.01); A01K 2217/052 (2013.01); A01K 2217/072 (2013.01); A01K 2217/15 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0331 (2013.01); A01K 2267/0381 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01); G01N 2333/70596 (2013.01)] 12 Claims
 
1. A genetically modified mouse whose genome comprises a replacement of a genomic fragment comprising exons 2-7 of a mouse CD47 gene at an endogenous mouse CD47 locus with a human genomic fragment comprising exons 2-7 of a human CD47 gene to form a humanized CD47 gene,
wherein the exons of the humanized CD47 gene consist of exon 1 of the mouse CD47 gene, exons 2-7 of the human CD47 gene, and the remaining exons downstream of exon 7 of the mouse CD47 gene,
wherein exons 2-7 of the human CD47 gene encode an amino acid sequence that is at least 98% identical with the amino acid sequence as set forth in amino acids 16-292 of SEQ ID NO: 10,
wherein the humanized CD47 gene is under control of the endogenous mouse CD47 promoter at the endogenous mouse CD47 locus, and
wherein the mouse expresses a humanized CD47 protein encoded by the humanized CD47 gene.