US 11,910,787 B2
Rodents having a humanized TMPRSS gene
Lisa Purcell, Garnerville, NY (US); Alexander O. Mujica, Elmsford, NY (US); and Yajun Tang, White Plains, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Nov. 17, 2020, as Appl. No. 17/099,942.
Application 17/099,942 is a division of application No. 16/052,700, filed on Aug. 2, 2018, granted, now 10,863,729.
Application 16/052,700 is a continuation of application No. 15/442,857, filed on Feb. 27, 2017, granted, now 10,070,632, issued on Sep. 11, 2018.
Claims priority of provisional application 62/301,023, filed on Feb. 29, 2016.
Prior Publication US 2021/0068377 A1, Mar. 11, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/47 (2006.01); C12N 15/09 (2006.01); C12N 15/63 (2006.01); C12N 9/64 (2006.01); C12N 5/0735 (2010.01); C12N 15/86 (2006.01); A61K 48/00 (2006.01)
CPC A01K 67/0275 (2013.01) [A01K 67/0278 (2013.01); C07K 14/47 (2013.01); C12N 5/0606 (2013.01); C12N 9/6424 (2013.01); C12N 15/63 (2013.01); C12N 15/86 (2013.01); A01K 2207/15 (2013.01); A01K 2217/072 (2013.01); A01K 2227/10 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0337 (2013.01); A61K 48/00 (2013.01); C07K 2319/00 (2013.01); C12N 15/09 (2013.01); C12N 2710/10332 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A rodent ES cell, comprising a replacement of a nucleotide sequence of an endogenous rodent Tmprss4 gene at an endogenous rodent Tmprss4 locus with a nucleotide sequence of a human TMPRSS4 gene to form a humanized Tmprss4 gene,
wherein the humanized Tmprss4 gene is under control of the promoter of the endogenous rodent Tmprss4 gene at the endogenous rodent Tmprss4 locus, and
wherein the humanized Tmprss4 gene encodes a humanized Tmprss4 protein that comprises (i) an ectodomain of the human TMPRSS4 protein encoded by the human TMPRSS4 gene, and (ii) a cytoplasmic and transmembrane portion encoded by the endogenous rodent Tmprss4 gene.