| CPC G06T 7/0012 (2013.01) [G06T 7/11 (2017.01); G06T 7/62 (2017.01); G06T 7/70 (2017.01)] | 20 Claims |

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1. A method comprising:
receiving, from a user device, one or more images, wherein each image, of the one or more images, was generated by application of a corresponding optical filter, of one or more optical filters, to light from a bioluminescent source located within a volume of biological material;
determining, based on the one or more images, one or more image segments;
generating one or more volume sub-regions by, for each volume sub-region, of the one or more volume sub-region, corresponding to an image segment of the one or more image segments:
generating one or more volume test-regions, wherein each of the one or more volume test-regions comprise one or more voxels assigned one or more radiation test-values, and wherein the one or more radiation test-values are determined based on the bioluminescent source, the volume of biological material, and the one or more optical filters;
generating, based on the one or more radiation test-values assigned to the one or more voxels and the one or more optical filters, one or more test images;
generating, based on the one or more test images, image test-segments;
determining correlation values between the image test-segments and the image segment; and
selecting, based on the correlation values of the image test-segments, a volume test-region as the volume sub-region;
determining, based on the one or more volume sub-regions, an output volume region comprising output voxels, wherein one or more output radiation values are assigned to each of the output voxels; and
causing image reconstruction, by a computing device and based on the output volume region, of the bioluminescent source.
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