US 12,236,583 B2
Three-dimensional luminescence imaging
Joshua Kempner, Medway, MA (US); and Jorge Ripoll, Madrid (ES)
Assigned to Revvity Health Sciences, Inc., Waltham, MA (US)
Filed by PerkinElmer Health Sciences, Inc., Waltham, MA (US)
Filed on Nov. 23, 2021, as Appl. No. 17/534,250.
Prior Publication US 2023/0162349 A1, May 25, 2023
Int. Cl. G06T 7/00 (2017.01); G06T 7/11 (2017.01); G06T 7/62 (2017.01); G06T 7/70 (2017.01)
CPC G06T 7/0012 (2013.01) [G06T 7/11 (2017.01); G06T 7/62 (2017.01); G06T 7/70 (2017.01)] 20 Claims
OG exemplary drawing
 
1. A method comprising:
receiving, from a user device, one or more images, wherein each image, of the one or more images, was generated by application of a corresponding optical filter, of one or more optical filters, to light from a bioluminescent source located within a volume of biological material;
determining, based on the one or more images, one or more image segments;
generating one or more volume sub-regions by, for each volume sub-region, of the one or more volume sub-region, corresponding to an image segment of the one or more image segments:
generating one or more volume test-regions, wherein each of the one or more volume test-regions comprise one or more voxels assigned one or more radiation test-values, and wherein the one or more radiation test-values are determined based on the bioluminescent source, the volume of biological material, and the one or more optical filters;
generating, based on the one or more radiation test-values assigned to the one or more voxels and the one or more optical filters, one or more test images;
generating, based on the one or more test images, image test-segments;
determining correlation values between the image test-segments and the image segment; and
selecting, based on the correlation values of the image test-segments, a volume test-region as the volume sub-region;
determining, based on the one or more volume sub-regions, an output volume region comprising output voxels, wherein one or more output radiation values are assigned to each of the output voxels; and
causing image reconstruction, by a computing device and based on the output volume region, of the bioluminescent source.