US 12,235,266 B2
Catalytic particles for signal enhancement for lateral flow immunoassays
David A. Kidwell, Alexandria, VA (US); and Shawn P. Mulvaney, Washington, DC (US)
Assigned to The Government of the United States of America, as represented by the Secretary of the Navy, Arlington, VA (US)
Filed by The Government of the United States of America, as represented by the Secretary of the Navy, Arlington, VA (US)
Filed on May 2, 2019, as Appl. No. 16/401,418.
Claims priority of provisional application 62/666,649, filed on May 3, 2018.
Prior Publication US 2019/0339265 A1, Nov. 7, 2019
Int. Cl. G01N 33/543 (2006.01); G01N 21/78 (2006.01); G01N 33/53 (2006.01); G01N 33/531 (2006.01); G01N 33/533 (2006.01); G01N 33/58 (2006.01); B82Y 99/00 (2011.01); G01N 21/77 (2006.01)
CPC G01N 33/54393 (2013.01) [G01N 21/78 (2013.01); G01N 33/5306 (2013.01); G01N 33/531 (2013.01); G01N 33/533 (2013.01); G01N 33/54346 (2013.01); G01N 33/583 (2013.01); G01N 33/587 (2013.01); B82Y 99/00 (2013.01); G01N 2021/7756 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A method for making catalytic particles for increased sensitivity in lateral flow immunoassays, comprising:
preparing a colloidal solution comprising water, a palladium salt, a protein, and sodium borohydride, wherein said colloidal solution is brown;
reducing the palladium salt in the presence of the protein to form a palladium-protein conjugate to act as a detecting agent in a lateral flow immunoassay;
using the sodium borohydride as a reducing agent in forming the palladium-protein conjugate to control particle size; and
forming brown or gray particles but not black particles, wherein the brown or gray particles are between 2 and 10 nm in size, and wherein the brown or gray particles catalytically develop a dye to detect the presence of an analyte when the analyte interacts with the protein.