US 12,235,264 B2
Enhanced cytometry for tissue characterization and screening
Nallakkan Arvindan, Moraga, CA (US); Stephen Mehi, San Francisco, CA (US); and Guanqing Ou, South San Francisco, CA (US)
Assigned to FIVE PRIME THERAPEUTICS, INC., South San Francisco, CA (US)
Appl. No. 17/058,108
Filed by FIVE PRIME THERAPEUTICS, INC., South San Francisco, CA (US)
PCT Filed May 23, 2019, PCT No. PCT/US2019/033755
§ 371(c)(1), (2) Date Nov. 23, 2020,
PCT Pub. No. WO2019/226897, PCT Pub. Date Nov. 28, 2019.
Claims priority of provisional application 62/676,620, filed on May 25, 2018.
Prior Publication US 2021/0215687 A1, Jul. 15, 2021
Int. Cl. G01N 33/543 (2006.01); B01F 23/50 (2022.01); G02B 13/22 (2006.01); G02B 15/00 (2006.01); G02B 21/00 (2006.01); G02B 21/16 (2006.01); G02B 21/26 (2006.01)
CPC G01N 33/54366 (2013.01) [B01F 23/50 (2022.01); G02B 13/22 (2013.01); G02B 15/00 (2013.01); G02B 21/0088 (2013.01); G02B 21/16 (2013.01); G02B 21/26 (2013.01); B01J 2219/00351 (2013.01); B01J 2219/00418 (2013.01)] 19 Claims
OG exemplary drawing
 
1. An optical imaging system, comprising:
a first stage comprising a sample holding region;
a frame configured to provide mechanical coupling between the first stage and a second stage,
wherein the first stage is configured to support a substrate comprising a plurality of microfluidic channels such that, in operation, the sample holding region holding a sample is located below the substrate supported by the first stage and a solution comprising fluorescently tagged probe molecules contacts the sample as the solution flows through the plurality of microfluidic channels;
one or more lenses disposed between the first stage and the second stage, the one or more lenses configured to receive light from the sample at the first stage, wherein an arrangement of the one or more lenses has a numerical aperture less than 0.1; and
a sensor array coupled to the second stage and configured to receive light passing through the one or more lenses, wherein the sensor array is configured to receive light passing through the one or more lenses to detect light fluorescing from the fluorescently tagged probe molecules.