US 12,235,262 B1
Methods and systems for single cell protein analysis
Paul Giresi, Palo Alto, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10X GENOMICS, INC., Pleasanton, CA (US)
Filed on Sep. 8, 2020, as Appl. No. 17/014,909.
Claims priority of provisional application 62/897,698, filed on Sep. 9, 2019.
Int. Cl. G01N 33/543 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/54306 (2013.01) [G01N 33/6842 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for processing a labelled cell, comprising:
(a) partitioning a plurality of labelled cells into a plurality of partitions, wherein a partition of the plurality of partitions comprises a cell barcode molecule and a labelled cell of the plurality of labelled cells, wherein the labelled cell comprises (i) a first labelling agent coupled to a first protein of the labelled cell, and (ii) a second labelling agent coupled to a second protein of the labelled cell, wherein the first labelling agent comprises a first proximity probe comprising a first barcode sequence and a first unique molecular identifier (UMI), wherein the second labelling agent comprises a second proximity probe comprising a second barcode sequence and a second UMI, and wherein the cell barcode molecule comprises a third UMI and a cell barcode sequence that is complementary to the first and second barcode sequences; and
(b) generating a barcoded molecule in the partition, wherein the barcoded molecule comprises the first barcode sequence, the first UMI, the second barcode sequence, the second UMI, the third UMI, and the cell barcode sequence, or derivatives thereof.