	US 12,235,261 B2
	Method for measuring cellular uptake of molecules
Yuki Noguchi, Shizuoka (JP); Takeru Nambu, Shizuoka (JP); Kazuhisa Ozeki, Shizuoka (JP); Noriaki Ohminato, Shizuoka (JP); Toshito Nakagawa, Shizuoka (JP); and Sotaro Naoi, Shizuoka (JP)
Assigned to CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo (JP)
Appl. No. 16/756,415
Filed by Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
PCT Filed Oct. 19, 2018, PCT No. PCT/JP2018/039005 § 371(c)(1), (2) Date Apr. 15, 2020, PCT Pub. No. WO2019/078357, PCT Pub. Date Apr. 25, 2019.
Claims priority of application No. 2017-203994 (JP), filed on Oct. 20, 2017.
Prior Publication US 2021/0190764 A1, Jun. 24, 2021
Int. Cl. G01N 33/50 (2006.01); G01N 33/49 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01)

CPC G01N 33/5035 (2013.01) [G01N 33/4915 (2013.01); G01N 33/5067 (2013.01); G01N 33/56966 (2013.01); G01N 33/582 (2013.01); G01N 2333/70589 (2013.01); G01N 2333/70596 (2013.01); G01N 2333/7155 (2013.01)]

13 Claims

1. A method for measuring a cellular uptake amount of a molecule, comprising the steps of:

(i) adding the molecule to an organ-derived cell population to perform incubation, wherein the molecule is incorporated into cells via a cell surface receptor,

(ii) sorting the organ-derived cell population based on expression levels of CD31 and CD45 to produce a cell population expressing CD31 and CD45, and

(iii) after steps (i) and (ii), measuring the amount of the molecule incorporated into the cell population,

wherein the method comprises step (ii) subsequent to step (i), and the organ-derived cell population incubated with the added molecule is sorted based on the expression levels of CD31 and CD45,

wherein the organ-derived cell population is a human hepatic nonparenchymal cell population, and

wherein a CD31^highCD45^lowcell population is sorted in step (ii).