US 12,235,260 B2
Nanopore-based analysis of analytes
Giovanni Maglia, Gilmmen (NL); Shengli Zhang, Shanghai (CN); and Andrew Heron, Maidenhead (GB)
Assigned to Rijksuniversiteit Groningen, Groningen (NL); and Portal Biotech Limited, Guildford (GB)
Filed by Rijksuniversiteit Groningen, Groningen (NL); and Portal Biotech Limited, Guildford (GB)
Filed on Dec. 22, 2023, as Appl. No. 18/394,975.
Application 18/394,975 is a continuation of application No. PCT/NL2023/050570, filed on Oct. 30, 2023.
Claims priority of application No. 22204590 (EP), filed on Oct. 28, 2022.
Prior Publication US 2024/0201165 A1, Jun. 20, 2024
Int. Cl. G01N 33/487 (2006.01)
CPC G01N 33/48721 (2013.01) 20 Claims
OG exemplary drawing
 
1. A method comprising:
(a) providing:
(i) a nanopore system, wherein the nanopore system comprises (1) a fluidic chamber, and (2) a membrane comprising a nanopore, wherein the membrane separates the fluidic chamber into a cis side and a trans side;
(b) contacting (1) a non-nucleic acid based polymer analyte, and (2) a translocase with the cis side of the nanopore; and
(c) translocating the non-nucleic acid based polymer analyte to the trans side of the fluidic chamber using an electro-osmotic force, wherein the electro-osmotic force acts from the cis side to the trans side, wherein the electro-osmotic force maintains the translocase at an entrance of a channel of the nanopore on the cis side of the fluidic chamber.