US 12,235,251 B2
Chromatography method, method of determining the concentration of at least one compound in a chromatography method, method of obtaining an adsorption isotherm, method of obtaining at least one stationary phase and method of evaluating the accuracy of a predetermined adsorption isotherm
Dominik Stein, Göttingen (DE); and Jan Schwellenbach, Göttingen (DE)
Assigned to Sartorius Stedim Biotech GmbH, (DE)
Appl. No. 17/613,701
Filed by Sartorius Stedim Biotech GmbH, Göttingen (DE)
PCT Filed May 8, 2020, PCT No. PCT/EP2020/062856
§ 371(c)(1), (2) Date Nov. 23, 2021,
PCT Pub. No. WO2020/239393, PCT Pub. Date Dec. 3, 2020.
Claims priority of application No. 19176446 (EP), filed on May 24, 2019; application No. 19176462 (EP), filed on May 24, 2019; and application No. 19176467 (EP), filed on May 24, 2019.
Prior Publication US 2022/0229029 A1, Jul. 21, 2022
Int. Cl. G01N 30/86 (2006.01); G01N 30/89 (2006.01)
CPC G01N 30/89 (2013.01) [G01N 30/8658 (2013.01); G01N 30/8675 (2013.01); G01N 30/8696 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method of obtaining an adsorption isotherm of at least one compound comprising the steps of:
(ia) selecting at least one compound;
(ib) selecting a stationary phase;
(ic) selecting a mobile phase;
(id) selecting a temperature of the at least one compound, the stationary phase and the mobile phase;
wherein at least one parameter, selected from parameters capable of characterizing the composition of the mobile phase and the temperature, varies;
(ii) obtaining a plurality of parameter value sets including the at least one varying parameter and the concentration of the at least one compound in the mobile phase;
(iii) obtaining a plurality of binding capacity values of the at least one compound by the stationary phase for each of the plurality of parameter value sets obtained in step (ii); and
(iv) obtaining the adsorption isotherm based on the plurality of binding capacity values obtained in step (iii) by means of multivariate data analysis,
wherein the adsorption isotherm obtained in step (iv) is an equation having the form q=f(c,pj) that relates the binding capacity q of a compound by the stationary phase to the concentration c of the at least one compound in the mobile phase and to at least one further parameter pj selected from parameters characterizing the composition of the mobile phase and the temperature.