| CPC G01N 21/78 (2013.01) [G01N 1/34 (2013.01)] | 14 Claims |
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1. A kit for quantification of porphobilinogen (PBG) in a urine sample, the kit comprising:
a) diagnostic test strip comprising:
a substrate impregnated with a dried solution comprising an acid, a reagent reactive with PBG, and one or more stabilizers;
b) a pre-processing reagent comprising urease,
wherein the amount of pre-processing reagent is sufficient to remove urea such that a 2 ml urine sample contacted with pre-processing reagent for a period of 10 seconds to 15 minutes will contain an amount of urea that is undetectable by the test strip; and
c) a cation-exchange resin device comprising a vessel and a cation-exchange resin arranged in the vessel,
wherein the cation-exchange resin binds to urobilinogen at an affinity greater than it binds to PBG, such that a urine sample of 2 ml contacted with the cation-exchange resin in the vessel for a period 5 to 20 minutes will contain an amount of urobilinogen that is undetectable by the test strip but will contain an amount of PBG detectable by the test strip.
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