US 12,235,215 B2
Processing and imaging tissue samples
Michael McLane, Menlo Park, CA (US)
Assigned to Akoya Biosciences, Inc., Menlo Park, CA (US)
Filed by Akoya Biosciences, Inc., Menlo Park, CA (US)
Filed on Jul. 20, 2021, as Appl. No. 17/380,631.
Claims priority of provisional application 63/054,226, filed on Jul. 20, 2020.
Prior Publication US 2022/0018778 A1, Jan. 20, 2022
Int. Cl. G01N 21/64 (2006.01); G01N 1/30 (2006.01); G06T 7/00 (2017.01)
CPC G01N 21/6456 (2013.01) [G01N 1/30 (2013.01); G01N 21/6428 (2013.01); G06T 7/0012 (2013.01); G01N 2001/302 (2013.01); G01N 2001/305 (2013.01); G01N 2021/6441 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/30024 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A method, comprising:
applying a first stain composition to a biological sample, and measuring information corresponding to one or more stains of the first stain composition in the biological sample, wherein the one or more stains comprise a fluorescent counterstain;
removing the fluorescent counterstain from the biological sample;
applying a second stain composition to the biological sample, and measuring information corresponding to one or more stains of the second stain composition in the biological sample, wherein the one or more stains comprise a fluorescent label;
generating a first image of the biological sample, wherein the first image corresponds to a pattern of simulated hematoxylin staining in the biological sample; and
generating a second image of the biological sample, wherein the second image corresponds to localization of the fluorescent label in the biological sample.